Induction of androgenesis and production of haploid embryos in anther cultures of borage (Borago officinalis L.)

被引:9
作者
Eshaghi, Zahra Chardoli [1 ]
Abdollahi, Mohammad Reza [1 ]
Moosavi, Sayyed Saeed [1 ]
Deljou, Ali [2 ]
Segui-Simarro, J. M. [3 ]
机构
[1] Bu Ali Sina Univ, Fac Agr, Dept Agron & Plant Breeding, Hamadan, Iran
[2] Bu Ali Sina Univ, Fac Agr, Dept Plant Biotechnol, Hamadan, Iran
[3] Univ Politecn Valencia, COMAV, E-46071 Valencia, Spain
基金
中国国家自然科学基金;
关键词
In vitro culture; Microspore embryogenesis; Plant growth regulators; Temperature treatment; ISOLATED MICROSPORE CULTURE; EMBRYOGENESIS INDUCTION; STAGE;
D O I
10.1007/s11240-015-0768-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Borage (Borago officinalis L.) is an important medicinal plant with different culinary, pharmaceutical and industrial properties. Unfortunately, there are no published reports on the establishment of protocols to produce DHs in this species up to now. In this work, we show for the first time the induction of borage microspores to become embryogenic calli, from which haploid embryos are produced. In addition, we evaluated the effect of using different flower bud sizes, carbon sources, concentrations of 2,4-D and BAP, cold (4 A degrees C) pretreatments and heat shock treatments. Production of total calli, embryogenic calli and callus-derived embryos was differently affected by the different parameters studied. Our results showed that the use of 5-7 mm-long flower buds, a cold (4 A degrees C) pretreatment during 4 days, a 32 A degrees C heat shock for 3 days, and the addition of 3 % maltose and 2 mgl(-1) 2,4-D and 1 mgl(-1) BAP to the culture medium, was beneficial for embryo production. Overall, this work demonstrates that DH technology is possible in borage, and opens the door for future improvements needed to finally obtain borage DH plants.
引用
收藏
页码:321 / 329
页数:9
相关论文
共 33 条
[1]  
ABDOLLAHI MR, 2004, GLOBAL IRAN J BIOTEC, V2, P97
[2]  
[Anonymous], REV ADV PLANT BIOTEC
[3]   ANTHER CULTURE AND ANDROGENETIC PLANT-REGENERATION IN BUCKWHEAT (FAGOPYRUM-ESCULENTUM MOENCH) [J].
BOHANEC, B ;
NESKOVIC, M ;
VUJICIC, R .
PLANT CELL TISSUE AND ORGAN CULTURE, 1993, 35 (03) :259-266
[4]  
Calleberg E, 1996, IN VITRO HAPLOID PRO, V23, P189
[5]  
Ciner Dudu Ozkum, 2002, Turkish Journal of Botany, V26, P131
[6]   TEMPERATURE CONTROLS BOTH GAMETOPHYTIC AND SPOROPHYTIC DEVELOPMENT IN MICROSPORE CULTURES OF BRASSICA-NAPUS [J].
CUSTERS, JBM ;
CORDEWENER, JHG ;
NOLLEN, Y ;
DONS, HJM ;
CAMPAGNE, MMV .
PLANT CELL REPORTS, 1994, 13 (05) :267-271
[7]  
Ferrie A M., 2013, Biotechnology for Medicinal Plants, P191
[8]   Microspore embryogenesis in Apiaceae [J].
Ferrie, A. M. R. ;
Bethune, T. D. ;
Mykytyshyn, M. .
PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 104 (03) :399-406
[9]   Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production [J].
Ferrie, A. M. R. ;
Caswell, K. L. .
PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 104 (03) :301-309
[10]   The resurgence of haploids in higher plants [J].
Forster, Brian P. ;
Heberle-Bors, Erwin ;
Kasha, Ken J. ;
Touraev, Alisher .
TRENDS IN PLANT SCIENCE, 2007, 12 (08) :368-375