Whole Genome Sequence Typing to Investigate the Apophysomyces Outbreak following a Tornado in Joplin, Missouri, 2011

被引：50

作者：

Etienne, Kizee A. ^{[1
]}

Gillece, John ^{[2
]}

Hilsabeck, Remy ^{[2
]}

Schupp, Jim M. ^{[2
]}

Colman, Rebecca ^{[2
]}

Lockhart, Shawn R. ^{[1
]}

Gade, Lalitha ^{[1
]}

Thompson, Elizabeth H. ^{[3
]}

Sutton, Deanna A. ^{[3
]}

Neblett-Fanfair, Robyn ^{[1
]}

Park, Benjamin J. ^{[1
]}

Turabelidze, George ^{[4
]}

Keim, Paul ^{[2
]}

Brandt, Mary E. ^{[1
]}

Deak, Eszter ^{[1
]}

Engelthaler, David M. ^{[2
]}

机构：

[1] Ctr Dis Control & Prevent, Atlanta, GA 30333 USA

[2] Translat Genom Res Inst, Flagstaff, AZ USA

[3] Univ Texas Hlth Sci Ctr San Antonio, Dept Pathol, San Antonio, TX 78229 USA

[4] Missouri Dept Hlth, St Louis, MO USA

来源：

PLOS ONE | 2012年 / 7卷 / 11期

关键词：

ELEGANS; ZYGOMYCOSIS; EPIDEMIOLOGY;

D O I：

10.1371/journal.pone.0049989

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces.

引用

页数：6

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