Na,K-ATPase alpha- and beta-isoforms in the developing cochlea of the mouse

被引:47
|
作者
Erichsen, S
Zuo, J
Curtis, L
Rarey, K
Hultcrantz, M
机构
[1] KAROLINSKA HOSP, DEPT OTORHINOLARYNGOL, S-17176 STOCKHOLM, SWEDEN
[2] UNIV FLORIDA, DEPT ANAT & CELL BIOL, GAINESVILLE, FL USA
[3] UNIV FLORIDA, DEPT OTOLARYNGOL, GAINESVILLE, FL USA
关键词
immunohistochemistry; inner ear; mouse; embryology; Na; K-ATPase; homeostasis; fluid and electrolyte;
D O I
10.1016/0378-5955(96)00105-0
中图分类号
R36 [病理学]; R76 [耳鼻咽喉科学];
学科分类号
100104 ; 100213 ;
摘要
Immunohistochemistry was used to investigate the presence of Na,K-ATPase alpha- and beta-subunits isoforms (alpha(1), alpha(2), alpha(3), beta(1) and beta(2)) in the cochlea of the mouse at different ages between embryological day (E) 19 and postnatal day (P)+30. alpha(1) was mainly found in the stria vascularis and in the spiral ligament; it increased steadily from p+4. These data correlates well with the morphological and electrophysiological maturation of the cochlea. alpha(3) predominated in the spiral ganglia and the cochlear nerve. This finding is well in accordance with reports that alpha(3) seems to be associated with the nervous system. The beta-subunit was found mainly in those tissues where staining of the alpha-subunit also was seen. Both subunits were localized in tissue regions where fluid regulation is expected to play an important role. For some isoforms, the expression pattern of Na,K-ATPase during development in the mouse is different from that in the rat. The expression of Na,K-ATPase and that of glucocorticoid receptors during development in the inner ear of the mouse show a similar pattern, which may indicate that glucocorticoid receptors could be involved in regulating the expression of Na,K-ATPase.
引用
收藏
页码:143 / 149
页数:7
相关论文
共 50 条
  • [31] Immunolabeling of alpha 3 isoforms of Na,K-ATPase trapped in endoplasmic reticulum of reticulocyte
    Chang, CS
    Lee, P
    Kirk, RG
    FASEB JOURNAL, 1996, 10 (03): : 3756 - 3756
  • [32] DISTRIBUTION OF ISOFORMS OF THE ALPHA-SUBUNIT OF THE NA,K-ATPASE IN ADULT-RAT
    ROWE, PM
    LINK, WT
    OSBORN, CP
    ALBERS, RW
    JOURNAL OF GENERAL PHYSIOLOGY, 1990, 96 (06): : A62 - A62
  • [33] Cellular and subcellular specification of Na,K-ATPase α and β isoforms in the postnatal development of mouse retina
    Wetzel, RK
    Arystarkhova, E
    Sweadner, KJ
    JOURNAL OF NEUROSCIENCE, 1999, 19 (22): : 9878 - 9889
  • [34] Sodium kinetics of Na,K-ATPase alpha isoforms in intact transfected HeLa cells
    Zahler, R
    Zhang, ZT
    Manor, M
    Boron, WF
    JOURNAL OF GENERAL PHYSIOLOGY, 1997, 110 (02): : 201 - 213
  • [35] Degradation and endoplasmic reticulum retention of unassembled alpha- and beta-subunits of Na,K-ATPase correlate with interaction of BiP
    Beggah, A
    Mathews, P
    Beguin, P
    Geering, K
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (34) : 20895 - 20902
  • [36] A major role for the Na,K-ATPase beta subunit isoforms in sorting and selective stabilization of the Na,K-ATPase in polarized gastric HGT-1 cells
    Vagin, O
    Turdikulova, S
    Sachs, G
    MOLECULAR BIOLOGY OF THE CELL, 2004, 15 : 434A - 434A
  • [37] Tuning of the Na,K-ATPase by the beta subunit
    Florian Hilbers
    Wojciech Kopec
    Toke Jost Isaksen
    Thomas Hellesøe Holm
    Karin Lykke-Hartmann
    Poul Nissen
    Himanshu Khandelia
    Hanne Poulsen
    Scientific Reports, 6
  • [38] Tuning of the Na,K-ATPase by the beta subunit
    Hilbers, Florian
    Kopec, Wojciech
    Isaksen, Toke Jost
    Holm, Thomas Hellesoe
    Lykke-Hartmann, Karin
    Nissen, Poul
    Khandelia, Himanshu
    Poulsen, Hanne
    SCIENTIFIC REPORTS, 2016, 6
  • [39] Expression of alpha isoforms of the Na-K-ATPase in the developing kidney.
    Aviles, DH
    Manning, J
    Vehaskari, VM
    PEDIATRIC RESEARCH, 1996, 39 (04) : 2130 - 2130
  • [40] IDENTIFYING A REGION OF ALPHA-/BETA-SUBUNIT INTERACTION ON THE GASTRIC H,K-ATPASE
    CHOW, DC
    OKAMOTO, CT
    MOSTOV, KE
    FORTE, JG
    MOLECULAR BIOLOGY OF THE CELL, 1995, 6 : 388 - 388