Study on Interaction of L-Homocysteine Modified Gold Nanoparticles with Bovine Serum Albumin by Fluorescence Spectroscopy

被引：1

作者：

Chen Hui-hui ^{[1
]}

Zhu Duan-xu ^{[1
]}

Guo Yan-li ^{[1
]}

Wang Ying ^{[1
]}

Yan Hong-tao ^{[1
]}

机构：

[1] NW Univ Xian, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710069, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2012年 / 32卷 / 12期

关键词：

Nanoparticles; Chemical modification; Bovine serum albumin; Fluorescence spectroscopy; BIOCOMPATIBILITY;

D O I：

10.3964/j.issn.1000-0593(2012)12-3276-05

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The interaction of L-homocysteine (Cys) modified gold nanoparticles with bovine serum albumin (BSA) was studied by fluorescence quenching spectroscopy and synchronous fluorescence spectra. The binding constant and binding sites of L-homocysteine modified gold nanoparticles to BSA were calculated, respectively, according to the double logarithm regression curve. Fluorescence quenching of BSA by L-homocysteine modified gold nanoparticles was observed, indicating that the quenching mechanism is static quenching. In addition, the thermodynamic data show that the key interaction force is hydrophobic interaction. Finally, the synchronous fluorescence spectra show that the conformation of BSA and the microenvironment of the tryptophan have not obviously changed.

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收藏

页码：3276 / 3280

页数：5

相关论文

共 15 条

[1] Spectroscopic identification of S-Au interaction in cysteine capped gold nanoparticles [J].

Aryal, S ;

Remant, BKC ;

Dharmaraj, N ;

Bhattarai, N ;

Kim, CH ;

Kim, HY .

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2006, 63 (01) :160-163

[2] Reversible and covalent binding of drugs to human serum albumin: Methodological approaches and physiological relevance [J].

Bertucci, C ;

Domenici, E .

CURRENT MEDICINAL CHEMISTRY, 2002, 9 (15) :1463-1481

[3] Study of protein-gold nanoparticle conjugates by fluorescence and surface-enhanced Raman scattering [J].

Iosin, M. ;

Toderas, F. ;

Baldeck, P. L. ;

Astilean, S. .

JOURNAL OF MOLECULAR STRUCTURE, 2009, 924 :196-200

[4] Dynamic Light Scattering as a Powerful Tool for Gold Nanoparticle Bioconjugation and Biomolecular Binding Studies [J].

Jans, Hilde ;

Liu, Xiong ;

Austin, Lauren ;

Maes, Guido ;

Huo, Qun .

ANALYTICAL CHEMISTRY, 2009, 81 (22) :9425-9432

[5] Binding of chloroquine-conjugated gold nanoparticles with bovine serum albumin [J].

Joshi, Prachi ;

Chakraborty, Soumyananda ;

Dey, Sucharita ;

Shanker, Virendra ;

Ansari, Z. A. ;

Singh, Surinder P. ;

Chakrabarti, Pinak .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2011, 355 (02) :402-409

[6] Gold nanoparticle-based sensing of "spectroscopically silent" heavy metal ions [J].

Kim, YJ ;

Johnson, RC ;

Hupp, JT .

NANO LETTERS, 2001, 1 (04) :165-167

[7] Gold nanoparticles induce oxidative damage in lung fibroblasts in vitro [J].

Li, Jasmine J. ;

Zou, Li ;

Hartono, Deny ;

Ong, Choon-Nam ;

Bay, Boon-Huat ;

Yung, Lin-Yue Lanry .

ADVANCED MATERIALS, 2008, 20 (01) :138-+

[8] Assessment of cytotoxicity of quantum dots and gold nanoparticles using cell-based. impedance spectroscopy [J].

Male, Keith B. ;

Lachance, Bernard ;

Hrapovic, Sabahudin ;

Sunahara, Geoff ;

Luong, John H. T. .

ANALYTICAL CHEMISTRY, 2008, 80 (14) :5487-5493

[9] Interaction of bovine serum albumin and albumin-gold nanoconjugates with L-aspartic acid. A spectroscopic approach [J].

Mandal, Gopa ;

Bardhan, Munmun ;

Ganguly, Tapan .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2010, 81 (01) :178-184

[10] THERMODYNAMICS OF PROTEIN ASSOCIATION REACTIONS - FORCES CONTRIBUTING TO STABILITY [J].

ROSS, PD ;

SUBRAMANIAN, S .

BIOCHEMISTRY, 1981, 20 (11) :3096-3102