Preimplantation genetic diagnosis for chromosome rearrangements - one blastomere biopsy versus two blastomere biopsy

被引：15
作者：
Brodie, D. [1 ]
Beyer, C. E. [1 ]
Osborne, E. [1 ]
Kralevski, V. [1 ]
Rasi, S. [1 ]
Osianlis, T. [1 ]
机构：
[1] Monash IVF, Clayton, Vic 3168, Australia
来源：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2012年 / 29卷 / 08期
关键词：
In vitro Fertilisation (IVF); Preimplantation Genetic Diagnosis (PGD); Fluorescent In Situ Hybridisation (FISH); Embryo biopsy; Translocation; CLEAVAGE-STAGE EMBRYOS; IMPLANTATION; PGD; CELLS; VIEW;
D O I：
10.1007/s10815-012-9782-2
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Preimplantation Genetic Diagnosis (PGD) has proven to be a useful reproductive option for carriers of some chromosome rearrangements. The data presented in this study compares the impact of one versus two blastomere biopsy on the likelihood of achieving a PGD result, as well as the effect on subsequent embryo development and clinical outcomes. IVF-PGD couples had either one or two blastomeres biopsied from all embryos with a parts per thousand yen7 blastomeres on day 3 post oocyte collection. These blastomeres were assessed for the specific chromosome rearrangement using Fluorescent In-situ Hybridisation (FISH). Further embryo development was monitored on days 4 and 5. Clinical outcomes were assessed retrospectively. The data shows that statistically more embryos achieved a PGD result following two blastomere biopsy, compared with one blastomere biopsy (92 % versus 88 %, respectively). Furthermore it was found that embryo development and clinical outcomes were similar between the two biopsy groups. Based on this analysis it appears that the biopsy of two blastomeres from embryos with a parts per thousand yen7 blastomeres on day 3 is a valid and successful approach for couples presenting for IVF-PGD for a chromosome rearrangement.
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收藏
页码：821 / 827
页数：7
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