Mimosine-Induced Apoptosis in C6 Glioma Cells Requires the Release of Mitochondria-Derived Reactive Oxygen Species and p38, JNK Activation

被引:14
作者
Qiao, Shanlou [1 ]
Murakami, Keiko [2 ]
Zhao, Qinghong [3 ]
Wang, Baoling [3 ]
Seo, Hisao [1 ]
Yamashita, Hitoshi [1 ]
Li, Xiaotao [4 ]
Iwamoto, Takashi [1 ]
Ichihara, Masatoshi [1 ]
Yoshino, Masataka [5 ]
机构
[1] Chubu Univ, Dept Biomed Sci, Coll Life & Hlth Sci, Aichi 4878501, Japan
[2] Aichi Med Univ, Dept Biochem, Sch Med, Nagakute, Aichi 4801195, Japan
[3] Nanjing Med Univ, Dept Surg, Affiliated Hosp 2, Nanjing 210011, Jiangsu, Peoples R China
[4] E China Normal Univ, Inst Biomed Sci, Shanghai 200241, Peoples R China
[5] Kinjo Gakuin Univ, Dept Food & Nutr Environm, Moriyama Ku, Nagoya, Aichi 4638521, Japan
关键词
C6 glioma cell; Mimosine; ROS; Apoptosis; Mitochondria; MALIGNANT GLIOMAS; OXIDATIVE STRESS; CYTOCHROME-C; IRON CHELATOR; PC12; CELLS; IN-VIVO; DEATH; DNA; INDUCTION; PATHWAYS;
D O I
10.1007/s11064-011-0628-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Growth-inhibitory effects of mimosine, a plant amino acid, on rat C6 glioma cells were analyzed. Mimosine markedly inhibited proliferation and induced apoptosis of C6 glioma cells in a dose- and time-dependent manner. Mimosine-mediated apoptosis was accompanied by promoting reactive oxygen species (ROS) generation in mitochondria, and by decreased mitochondrial membrane potential (Delta psi), and release of cytochrome c from mitochondria, followed by caspase 3 activation. Furthermore, mimosine increased the phosphorylation level of c-Jun-N-terminal protein kinase and p38, which was the downstream effect of ROS accumulation. Mimosine was confirmed to show profound effects on apoptosis of C6 glioma cells by ROS-regulated mitochondria pathway, and these results bear on the hypothesized potential for mimosine as promising agents in the treatment of malignant gliomas.
引用
收藏
页码:417 / 427
页数:11
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