An ellipsometric biosensor using aptamer for the detection of mercuric ions

被引：4

作者：

Ertan, Tugce ^{[1
]}

Caglayan, Mustafa Oguzhan ^{[1
,2
]}

机构：

[1] Sivas Cumhuriyet Univ, Chem Engn Dept, Sivas, Turkey

[2] Bilecik Seyh Edebali Univ, Dept Bioengn, Bilecik, Turkey

来源：

CHEMICAL PAPERS | 2021年 / 75卷 / 01期

关键词：

DNA aptamer; Ellipsometry; Biosensor; Mercuric ion; SENSITIVE DETECTION; IN-VITRO; SENSOR; MOLECULES; HG2+; MERCURY(II); TOXICOLOGY; SELECTION; SURFACES; PROBE;

D O I：

10.1007/s11696-020-01282-3

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An ellipsometric biosensor, to be used in the detection of mercuric ions that affect the vital organs of living organisms, was developed. In this context, as a diagnostic element of the developed ellipsometric biosensor, a nucleic acid-based aptamer that is highly selective for mercuric ions (Hg2+) was used. Two different aptamers, a long and straight sequence, and a hairpin-structured oligonucleotide sequence were chosen. The detection sensitivity obtained by both aptamers was 0.237 degrees/mu M and 0.211 degrees/mu M, respectively. The detection limit was 0.89 pM Hg2+ and 187 nM Hg2+ for linear- and hairpin-type oligonucleotide, respectively.

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页码：89 / 97

页数：9

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