Impact of automation on mass spectrometry

被引:35
作者
Zhang, Yan Victoria [1 ]
Rockwood, Alan [2 ,3 ]
机构
[1] Univ Rochester, Med Ctr, Dept Pathol & Lab Med, Rochester, NY 14642 USA
[2] Univ Utah, Sch Med, Dept Pathol, Salt Lake City, UT 84112 USA
[3] ARUP Labs, Salt Lake City, UT 84108 USA
关键词
Mass spectrometry; Liquid chromatography; Clinical practices; Automation; Routine clinical laboratories; High throughput; BAR-CODE TECHNOLOGY; ANTITHYROGLOBULIN AUTOANTIBODIES; ELECTROSPRAY IONIZATION; 25-HYDROXYVITAMIN D-2; FORENSIC TOXICOLOGY; INBORN-ERRORS; ASSAY; THYROGLOBULIN; THROUGHPUT; SERUM;
D O I
10.1016/j.cca.2015.08.027
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Mass spectrometry coupled to liquid chromatography (LC-MS and LC-MS/MS) is an analytical technique that has rapidly grown in popularity in clinical practice. In contrast to traditional technology, mass spectrometry is superior in many respects including resolution, specificity, multiplex capability and has the ability to measure analytes in various matrices. Despite these advantages, LC-MS/MS remains high cost, labor intensive and has limited throughput. This specialized technology requires highly trained personnel and therefore has largely been limited to large institutions, academic organizations and reference laboratories. Advances in automation will be paramount to break through this bottleneck and increase its appeal for routine use. This article reviews these challenges, shares perspectives on essential features for LC-MS/MS total automation and proposes a step-wise and incremental approach to achieve total automation through reducing human intervention, increasing throughput and eventually integrating the LC-MS/MS system into the automated clinical laboratory operations. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:298 / 303
页数:6
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