Combination of micro-fluidic chip with fluorescence correlation Spectroscopy for single molecule detection

被引：0
|
作者：
Bi, R [1 ]
Zhang, PD [1 ]
Dong, CQ [1 ]
Ren, JC [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2006年 / 17卷 / 04期
关键词：
fluorescence correlation spectroscopy; micro-fluidic chip; single molecule detection;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A single molecule detection technique was developed by the combination of a single channel poly (dimethylsiloxane)/glass micro-fluidic chip and fluorescence correlation spectroscopy (FCS). This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.
引用
收藏
页码：521 / 524
页数：4
相关论文
共 50 条
  • [41] Reversible photoswitching enables single-molecule fluorescence fluctuation Spectroscopy at high molecular concentration
    Eggeling, C.
    Hilbert, M.
    Bock, H.
    Ringemann, C.
    Hofmann, M.
    Stiel, A. C.
    Andresen, M.
    Jakobs, S.
    Egner, A.
    Schoenle, A.
    Hell, S. W.
    MICROSCOPY RESEARCH AND TECHNIQUE, 2007, 70 (12) : 1003 - 1009
  • [42] Early detection of apoptosis in living cells by fluorescence correlation spectroscopy
    Michelle M. Martinez
    Randall D. Reif
    Dimitri Pappas
    Analytical and Bioanalytical Chemistry, 2010, 396 : 1177 - 1185
  • [43] Single C-Reactive Protein Molecule Detection on a Gold-Nanopatterned Chip Based on Total Internal Reflection Fluorescence
    Heo, Yunmi
    Lee, Seungah
    Lee, Sang-Won
    Kang, Seong Ho
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2013, 34 (09): : 2725 - 2730
  • [44] Counting nucleosomes in living cells with a combination of fluorescence correlation spectroscopy and confocal imaging
    Weidemann, T
    Wachsmuth, M
    Knoch, TA
    Müller, G
    Waldeck, W
    Langowski, J
    JOURNAL OF MOLECULAR BIOLOGY, 2003, 334 (02) : 229 - 240
  • [45] Simulation of a stream of photon counts in single-molecule fluorescence fluctuation spectroscopy
    I. P. Shingaryov
    V. V. Skakun
    V. V. Apanasovich
    Journal of Applied Spectroscopy, 2012, 78 : 892 - 898
  • [46] Strategies to reduce detection volume of fluorescence correlation spectroscopy (FCS) to realize physiological concentration measurements
    Li, Jialing
    Dong, Chaoqing
    Ren, Jicun
    TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2017, 89 : 181 - 189
  • [47] Single-molecule detection by two-photon excitation of fluorescence
    Zander, C
    Brand, L
    Eggeling, C
    Drexhage, KH
    Seidel, CAM
    ADVANCES IN FLUORESCENCE SENSING TECHNOLOGY III, 1997, 2980 : 552 - 558
  • [48] Single molecule fluorescence burst detection of DNA separated by capillary electrophoresis
    Haab, BB
    Mathies, RA
    FLUORESCENCE DETECTION IV, PROCEEDINGS OF, 1996, 2705 : 162 - 169
  • [49] Plasmon-Controlled Fluorescence: Applications to Sensing and Single Molecule Detection
    Fu, Yi
    Zhang, Jian
    Lakowicz, Joseph R.
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES X, 2010, 7569
  • [50] Exploring the physics of single molecules of charged macromolecules by fluorescence correlation spectroscopy
    Luo, Shuangjiang
    Yang, Qingbo
    Jia, Pengxiang
    Wang, Shengqin
    Yang, Jingfa
    Zhao, Jiang
    SINGLE MOLECULE SPECTROSCOPY AND SUPERRESOLUTION IMAGING VI, 2013, 8590
12345