Combination of micro-fluidic chip with fluorescence correlation Spectroscopy for single molecule detection

被引:0
|
作者
Bi, R [1 ]
Zhang, PD [1 ]
Dong, CQ [1 ]
Ren, JC [1 ]
机构
[1] Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China
关键词
fluorescence correlation spectroscopy; micro-fluidic chip; single molecule detection;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A single molecule detection technique was developed by the combination of a single channel poly (dimethylsiloxane)/glass micro-fluidic chip and fluorescence correlation spectroscopy (FCS). This method was successfully used to determine the proportion of two model components in the mixture containing fluorescein and the rhodamine-green succinimidyl ester.
引用
收藏
页码:521 / 524
页数:4
相关论文
共 50 条
  • [31] Ligand binding of a ribosome-displayed protein detected in solution at the single molecule level by fluorescence correlation spectroscopy
    Jermutus, L
    Kolly, R
    Földes-Papp, Z
    Hanes, J
    Rigler, R
    Plückthun, A
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2002, 31 (03): : 179 - 184
  • [32] Single gold nanoparticles to enhance the detection of single fluorescent molecules at micromolar concentration using fluorescence correlation spectroscopy
    Punj, Deep
    Rigneault, Herve
    Wenger, Jerome
    NANOPHOTONICS V, 2014, 9126
  • [33] Hierarchical organization of the plasma membrane: Investigations by single-molecule tracking vs. fluorescence correlation spectroscopy
    Kusumi, Akihiro
    Shirai, Yuki M.
    Koyama-Honda, Ikuko
    Suzuki, Kenichi G. N.
    Fujiwara, Takahiro K.
    FEBS LETTERS, 2010, 584 (09) : 1814 - 1823
  • [34] Systematic characterization of extracellular vesicles sorting domains and quantification at the single molecule - single vesicle level by fluorescence correlation spectroscopy and single particle imaging
    Corso, Giulia
    Heusermann, Wolf
    Trojer, Dominic
    Goergens, Andre
    Steib, Emmanuelle
    Voshol, Johannes
    Graff, Alexandra
    Genoud, Christel
    Lee, Yi
    Hean, Justin
    Nordin, Joel Z.
    Wiklander, Oscar P. B.
    El Andaloussi, Samir
    Meisner-Kober, Nicole
    JOURNAL OF EXTRACELLULAR VESICLES, 2019, 8 (01)
  • [35] Second harmonic generation correlation spectroscopy for single molecule experiments
    Liu, Jing
    Irudayaraj, Joseph
    OPTICS EXPRESS, 2013, 21 (22): : 27063 - 27073
  • [36] Single molecule FCS-based oxygen sensor (O2-FCSensor):: a new intrinsically calibrated oxygen sensor utilizing fluorescence correlation spectroscopy (FCS) with single fluorescent molecule detection sensitivity
    Opitz, N
    Rothwell, PJ
    Oeke, B
    Schwille, PS
    SENSORS AND ACTUATORS B-CHEMICAL, 2003, 96 (1-2) : 460 - 467
  • [37] Applications of Fluorescence Correlation Spectroscopy in the Study of Single Chains of Polymers
    Zhou, Chao
    Yang, Jing-fa
    Zhao, Jiang
    ACTA POLYMERICA SINICA, 2021, 52 (03): : 321 - 334
  • [38] Early detection of apoptosis in living cells by fluorescence correlation spectroscopy
    Martinez, Michelle M.
    Reif, Randall D.
    Pappas, Dimitri
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 396 (03) : 1177 - 1185
  • [39] FLUORESCENCE CORRELATIONS, SINGLE-MOLECULE DETECTION AND LARGE NUMBER SCREENING - APPLICATIONS IN BIOTECHNOLOGY
    RIGLER, R
    JOURNAL OF BIOTECHNOLOGY, 1995, 41 (2-3) : 177 - 186
  • [40] Highly sensitive detection of DNA fragments by fluorescence correlation spectroscopy
    Shao, Wenchong
    Dong, Shiqing
    Tang, Xiaoqiong
    Chen, Jianling
    Yang, Hongqin
    OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS X, 2020, 11553