The Arabidopsis METACASPASE9 Degradome

被引：95

作者：

Tsiatsiani, Liana ^{[1
,2
,3
,4
]}

Timmerman, Evy ^{[3
,4
]}

De Bock, Pieter-Jan ^{[3
,4
]}

Vercammen, Dominique ^{[1
,2
]}

Stael, Simon ^{[1
,2
,3
,4
]}

van de Cotte, Brigitte ^{[1
,2
]}

Staes, An ^{[3
,4
]}

Goethals, Marc ^{[3
,4
]}

Beunens, Tine ^{[1
,2
]}

Van Damme, Petra ^{[3
,4
]}

Gevaert, Kris ^{[3
,4
]}

Van Breusegem, Frank ^{[1
,2
]}

机构：

[1] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium

[3] Univ Ghent VIB, Dept Med Prot Res, B-9000 Ghent, Belgium

[4] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium

来源：

PLANT CELL | 2013年 / 25卷 / 08期

关键词：

PROGRAMMED CELL-DEATH; LEISHMANIA-MAJOR METACASPASE; PHOSPHOENOLPYRUVATE CARBOXYKINASE; TRYPANOSOMA-BRUCEI; PLANT PROTEASES; COORDINATE REGULATION; CYSTEINE PROTEASES; YEAST METACASPASE; IN-VIVO; PROTEIN;

D O I：

10.1105/tpc.113.115287

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metacaspases are distant relatives of the metazoan caspases, found in plants, fungi, and protists. However, in contrast with caspases, information about the physiological substrates of metacaspases is still scarce. By means of N-terminal combined fractional diagonal chromatography, the physiological substrates of METACASPASE9 (MC9; AT5G04200) were identified in young seedlings of Arabidopsis thaliana on the proteome-wide level, providing additional insight into MC9 cleavage specificity and revealing a previously unknown preference for acidic residues at the substrate prime site position P1'. The functionalities of the identified MC9 substrates hinted at metacaspase functions other than those related to cell death. These results allowed us to resolve the substrate specificity of MC9 in more detail and indicated that the activity of phosphoenolpyruvate carboxykinase 1 (AT4G37870), a key enzyme in gluconeogenesis, is enhanced upon MC9-dependent proteolysis.

引用

页码：2831 / 2847

页数：17

共 86 条

[1]

Abramoff M.D., 2004, Biophotonics International, V11, P36

[2] Global kinetic analysis of proteolysis via quantitative targeted proteomics [J].

Agard, Nicholas J. ;

Mahrus, Sami ;

Trinidad, Jonathan C. ;

Lynn, Aenoch ;

Burlingame, Alma L. ;

Wells, James A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (06) :1913-1918

[3] An essential role for the Leishmania major metacaspase in cell cycle progression [J].

Ambit, A. ;

Fasel, N. ;

Coombs, G. H. ;

Mottram, J. C. .

CELL DEATH AND DIFFERENTIATION, 2008, 15 (01) :113-122

[4] Classification of the caspase-hemoglobinase fold: Detection of new families and implications for the origin of the eukaryotic separins [J].

Aravind, L ;

Koonin, EV .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2002, 46 (04) :355-367

[5] The domains of death: evolution of the apoptosis machinery [J].

Aravind, L ;

Dixit, VM ;

Koonin, EV .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (02) :47-53

[6] Cysteine proteases XCP1 and XCP2 aid micro-autolysis within the intact central vacuole during xylogenesis in Arabidopsis roots [J].

Avci, Utku ;

Petzold, H. Earl ;

Ismail, Ihab O. ;

Beers, Eric P. ;

Haigler, Candace H. .

PLANT JOURNAL, 2008, 56 (02) :303-315

[7] Coordinate regulation of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase by light and CO2 during C4 photosynthesis [J].

Bailey, Karen J. ;

Gray, Julie E. ;

Walker, Robert P. ;

Leegood, Richard C. .

PLANT PHYSIOLOGY, 2007, 144 (01) :479-486

[8] Metacaspase activity of Arabidopsis thaliana is regulated by S-nitrosylation of a critical cysteine residue [J].

Belenghi, Beatrice ;

Romero-Puertas, Maria C. ;

Vercammen, Dominique ;

Brackenier, Anouk ;

Inze, Dirk ;

Delledonne, Massimo ;

Van Breusegem, Frank .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (02) :1352-1358

[9] Post mortem function of AtMC9 in xylem vessel elements [J].

Bollhoner, Benjamin ;

Zhang, Bo ;

Stael, Simon ;

Denance, Nicolas ;

Overmyer, Kirk ;

Goffner, Deborah ;

Van Breusegem, Frank ;

Tuominen, Hannele .

NEW PHYTOLOGIST, 2013, 200 (02) :498-510

[10] What happened to plant caspases? [J].

Bonneau, Laurent ;

Ge, Yuan ;

Drury, Georgina E. ;

Gallois, Patrick .

JOURNAL OF EXPERIMENTAL BOTANY, 2008, 59 (03) :491-499

← 1 2 3 4 5 6 7 8 9 →