Stimulus-Responsive, Biodegradable, Biocompatible, Covalently Cross-Linked Hydrogel Based on Dextrin and Poly(N-isopropylacrylamide) for in Vitro/in Vivo Controlled Drug Release

被引:120
作者
Das, Dipankar [1 ]
Ghosh, Paulomi [2 ]
Ghosh, Animesh [3 ]
Haldar, Chanchal [1 ]
Dhara, Santanu [2 ]
Panda, Asit Baran [4 ]
Pal, Sagar [1 ]
机构
[1] Indian Sch Mines, Dept Appl Chem, Polymer Chem Lab, Dhanbad 826004, Bihar, India
[2] Indian Inst Technol, Sch Med Sci & Technol, Biomat & Tissue Engn Lab, Kharagpur 721302, W Bengal, India
[3] Birla Inst Technol, Dept Pharmaceut Sci, Ranchi 835215, Jharkhand, India
[4] CSIR, Cent Salt & Marine Chem Res Inst, Discipline Inorgan Mat & Catalysis, Bhavnagar 364002, Gujarat, India
关键词
biodegradable; noncytotoxicity; controlled drug release; dextrin; hydrogel; NANOCOMPOSITE HYDROGELS; MECHANICAL-PROPERTIES; GRAFT-COPOLYMERS; POROUS HYDROGEL; DELIVERY; CHITOSAN; PH; NANOPARTICLES; MICROSPHERES; ADHESION;
D O I
10.1021/acsami.5b02975
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
A novel stimulus-sensitive covalently cross-linked hydrogel derived from dextrin, N-isopropylacrylamide, and N,N'-methylene bis(acrylamide) (c-Dxt/pNIPAm), has been synthesized via Michael type addition reaction for controlled drug release application. The chemical structure of c-Dxt/pNIPAm has been confirmed through Fourier transform infrared (FTIR) spectroscopy and H-1 and C-13 NMR spectral analyses. The surface morphology of the hydrogel has been studied by field emission scanning electron microscopic (FE-SEM) and environmental scanning electron microscopic (E-SEM) analyses. The stimulus responsiveness of the hydrogel was studied through equilibrium swelling in various pH media at 25 and 37 degrees C. Rheological study was performed to measure the gel strength and gelation time. Noncytotoxicity of c-Dxt/pNIPAm hydrogel has been studied using human mesenchymal stem cells (hMSCs). The biodegradability of c-Dxt/pNIPAm was confirmed using hen egg lysozyme. The in vitro and in vivo release studies of ornidazole and ciprofloxacin imply that c-Dxt/pNIPAm delivers both drugs in a controlled way and would be an excellent alternative for a dual drug carrier. The FTIR, powder X-ray diffraction (XRD), and UV vis near infrared (NIR) spectra along with the computational study predict that the drugs remain in the matrix through physical interaction. A stability study signifies that the drugs (ornidazole similar to 97% and ciprofloxacin similar to 98%) are stable in the tablet formulations for up to 3 months.
引用
收藏
页码:14338 / 14351
页数:14
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