Thioredoxin negatively regulates p38 MAP kinase activation and IL-6 production by tumor necrosis factor-α

被引：63
作者：
Hashimoto, S
Matsumoto, K
Gon, Y
Furuichi, S
Maruoka, S
Takeshita, I
Hirota, K
Yodoi, J
Horie, T
机构：
[1] Nihon Univ, Sch Med, Dept Internal Med 1, Itabashi Ku, Tokyo 1738610, Japan
[2] Kyoto Univ Hosp, Dept Anesthesia, Kyoto, Japan
[3] Kyoto Univ, Inst Virus Res, Dept Biol Responses, Kyoto 606, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 258卷 / 02期
关键词：
D O I：
10.1006/bbrc.1999.0658
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We examined the regulatory role of a reduction/oxidation (redox) control protein, thioredoxin (TRX), in tumor necrosis factor-alpha (TNF-alpha)-induced p38 MAP kinase activation and p38 MAP kinase-mediated cytokine expression utilizing TRX-transfected murine L929 cells (TRX14). The results showed that TNF-alpha-induced p38 MAP kinase activation and interleukin-6 (IL-6) production by TRX 14 were less than those by the parental L cells and the control transfected L cells (Neo-1). SB 203580 as the specific inhibitor for p38 MAP kinase activity inhibited TNF-alpha-induced IL-6 production by the parental L cells, indicating that TNF-alpha-activated p38 MAP kinase regulates IL-6 production by the cell lines used in this study. These results showed that overexpression of TRX negatively regulates p38 MAP kinase activation and p38 MAP kinase-mediated IL-6 production by TNF-alpha-stimulated cells, indicating that TRX is critical for p38 MAP kinase activation which regulates cytokine expression. (C) 1999 Academic Press.
引用
收藏
页码：443 / 447
页数：5
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