Comparison of SYBR Green I and TaqMan real-time PCR formats for the analysis of her2 gene dose in human breast tumors

被引：13
作者：
Matsenko, N. U. [1 ]
Rijikova, V. S. [1 ]
Kovalenko, S. P. [1 ]
机构：
[1] Russian Acad Med Sci, Siberian Div, Inst Mol Biol & Biophys, Lab Gene Engn Methods, Moscow 109801, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2008年 / 145卷 / 02期
关键词：
her2; SYBR Green I; TaqMan; breast cancer;
D O I：
10.1007/s10517-008-0060-3
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We compared two technologies of real-time PCR (with the use of fluorescent SYBR Green I dye and specific TaqMan probe) for quantification of the dose of her2 gene in breast tumors. The maximum increase in the gene dose in TaqMan and SYBR Green I analyses was 10-and 5-fold, respectively. In was found that TaqMan and SYBR Green I technologies allow detection of the matrix in amounts corresponding to 1-100 and 2.5-40.0 ng genomic DNA, respectively. Tenfold increase in the gene dose leads to incorrect evaluation of multiplication ratio in the SYBR Green I analysis. These results suggest that TaqMan technology is more preferable for correct evaluation of her2 gene dose.
引用
收藏
页码：240 / 244
页数：5
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