The effect of polar groups on structural characteristics of phospholipid monolayers spread at the air-water interface

被引:104
作者
Minones, J
Patino, JMR
Conde, O
Carrera, C
Seoane, R
机构
[1] Univ Santiago de Compostela, Fac Pharm, Dept Phys Chem, Santiago De Compostela 15706, Spain
[2] Univ Sevilla, Fac Chem, Dept Chem Engn, E-41012 Seville, Spain
关键词
phospholipid monolayer; monolayer structures; Brewster angle microscopy;
D O I
10.1016/S0927-7757(01)01107-4
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Structural characteristics (structure, morphology, and relative film thickness) of dipalmitoyl phosphatidylcholine (DPPC), dipalmitoyl phosphatidylglycerol (DPPG) and dipalmitoyl phosphatidylserine (DPPS) monolayers were determined at the air-water interface at 20 degreesC and at pH 6 by means of surface pressure (pi)-area (A) isotherms coupled with Brewster angle microscopy (BAM). At lower surface pressures, phospholipid monolayers adopted an expanded-homogeneous structure at the air-water interface. As the surface pressure increases, in the liquid-condensed phase (LC), phospholipid monolayers showed film anisotropy and domains with heterogeneous structures. The homogeneous structures observed at higher surface pressures proved the existence of parallel oriented aliphatic chains when the close-packed film molecules were in the solid state. The relative monolayer thickness increased with the surface pressure and was at a maximum at the collapse point. The phospholipid head-group has an important role on the structural characteristics of the monolayer at the air-water interface. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:273 / 286
页数:14
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