Single-molecule interactions studied by using a modified DNA sequencer: A comparison with surface plasmon resonance data

被引：1

作者：

Sobek, Jens ^{[1
]}

Schauer, Stefan ^{[1
]}

Rehrauer, Hubert ^{[1
]}

Fischer, David ^{[1
]}

Patrignani, Andrea ^{[1
]}

Landgraf, Stephan ^{[2
]}

Schlapbach, Ralph ^{[1
]}

机构：

[1] Univ Zurich, Funct Genom Ctr Zurich, Swiss Fed Inst Technol, Winterthurerstr 190, CH-8057 Zurich, Switzerland

[2] Graz Univ Technol, Inst Phys & Theoret Chem, A-8010 Graz, Austria

来源：

REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS V | 2013年 / 8596卷

关键词：

Single-molecule study; zero-mode waveguide technology; fluorescence; hybridization; surface plasmon resonance; biomolecular interaction; short oligonucleotides; dye effect; POLYMERASE MOLECULES; BIOSENSOR ANALYSIS; FLUORESCENCE;

D O I：

10.1117/12.2002953

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this study we established the principles for using a commercial off-the-shelf DNA sequencer (RS, Pacific Biosciences) to measure biomolecular interactions. 1,2 Binding between single oligonucleotides immobilized at the bottom of a nanowell to a complementary DNA strand was used as a model system. The influence of the labeling dye on the hybridization of a 7mer oligonucleotide was investigated. The resulting association and dissociation rate constants (k(on) and k(off)) and the calculated dissociation constants (K-d) were compared to data obtained from surface plasmon resonance (SPR) measurements. In the present study we identified a good agreement of the determined kinetic constants by the two methods investigated.

引用

页数：10

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