Powering mammalian genetic screens with mouse haploid embryonic stem cells

被引：4

作者：

Zhang, Shiqiang ^{[1
,2
]}

Teng, Yue ^{[3
]}

机构：

[1] NW A&F Univ, Coll Vet Med, Dept Anim Biotechnol, Yangling 712100, Shaanxi, Peoples R China

[2] Shaanxi Ctr Stem Cell Engn & Technol, Yangling, Shaanxi, Peoples R China

[3] Arizona State Univ, Sch Biol & Hlth Syst Engn, Tempe, AZ 85287 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2013年 / 741卷

关键词：

Haploid embryonic stem cells; Mouse; Forward genetics; Reverse genetics; GENERATION; DISRUPTION; DERIVATION; OOCYTES;

D O I：

10.1016/j.mrfmmm.2013.01.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Generating homozygous mutants in mammalian cells has been complicated by their diploid genome. If one allele of an autosomal gene was disrupted, the resulting heterozygous mutant is unlikely to display a phenotype due to the existence of the other allele. Although embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are excellent cellular models for analyzing developmental events or disease phenotypes in vitro, a direct analysis of recessive phenotypes has been limited by their diploidy. Recently, four independent research groups reported successful derivation of haploid mouse embryonic stem cells which provide an effective platform for studying mammalian gene function. The aim of this review is to describe the strategies for deriving haploid ESCs and compare their characteristics with diploid ESCs, and further discuss the potential application of haploid ESCs in genetic screening and homozygous mutant animal production. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：44 / 50

页数：7

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