Recombinant l-phenylalanine ammonia lyase from Rhodosporidium toruloides as a potential anticancer agent

被引：31

作者：

Babich, Olga O. ^{[1
]}

Pokrovsky, Vadim S. ^{[2
,3
]}

Anisimova, Natalia Yu. ^{[3
]}

Sokolov, Nikolai N. ^{[2
]}

Prosekov, Alexander Yu. ^{[1
]}

机构：

[1] Kemerovo Technol Inst Food Ind, Kemerovo, Russia

[2] Russian Acad Med Sci, VN Orekhovich Inst Biomed Chem, Moscow, Russia

[3] Russian Acad Med Sci, NN Blokhin Russian Canc Res Ctr, Moscow, Russia

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2013年 / 60卷 / 03期

关键词：

Rhodosporidium toruloides; l-phenylalanine ammonia lyase; anticancer activity; cytotoxic enzyme; recombinant enzyme; L-ASPARAGINASE; EXPRESSION; CLONING;

D O I：

10.1002/bab.1089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recombinant producer strain expressing Rhodosporidium toruloides l-phenylalanine ammonia lyase (PAL) has been obtained, and a purification procedure of PAL has been developed. The purified enzyme, PAL, has the following biochemical and catalytic characteristics: Km for l-Phe of 0.49 mM, pH optimum at 8.5, and temperature optimum at 50 degrees C. PAL exhibited a significant cytotoxic effect toward the following cell lines: MCF7 (IC50 = 1.97 U/mL), DU145 (IC50 = 7.3 U/mL), which are comparable with E. coli l-asparaginase type-II cytotoxicity in vitro. Administration of PAL (200-400 U/kg) to L5178y-bearing mice for five times (a total dose of 1000-2000 U/kg) was well tolerated and showed the increase of life span (ILS) = 12-16%, P < 0.05. Data obtained suggest that PAL from R. toruloides has a potential for cancer treatment.

引用

页码：316 / 322

页数：7

共 24 条

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[2]

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