Characterization of type IV antifreeze gene in Nile tilapia (Oreochromis niloticus) and influence of cold and hot weather on its expression and some immune-related genes

被引:12
作者
Ammar, Asmma Y. [1 ]
El Nahas, Abeer F. [2 ]
Mahmoud, Shawky [3 ]
Barakat, Mohamed E. [4 ]
Hassan, Asmaa M. [1 ]
机构
[1] Anim Hlth Res Inst, Dept Biotechnol, Kafr Al Sheikh, Egypt
[2] Alexandria Univ, Fac Vet Med, Anim Husb & Anim Wealth Dept, Edfina 22758, Behera, Egypt
[3] Kafer El Sheikh Univ, Dept Physiol, Fac Vet Med, Kafr Al Sheikh, Egypt
[4] Anim Hlth Res Inst, Dept Biotechnol, Kafer El Sheik, Egypt
关键词
Oreochromis niloticus; Antifreeze gene; Immune-related genes; Thermal stress; APOLIPOPROTEIN-A-I; MACROZOARCES-AMERICANUS; ATLANTIC SALMON; TELEOST FISH; COMMON CARP; OCEAN POUT; POLAR FISH; PROTEIN; TEMPERATURE; GROWTH;
D O I
10.1007/s10695-017-0450-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this work is to study the effect of the thermal stress of ambient temperature during winter and summer on the expression of type IV antifreeze gene (ANF IV) in different tissues of Nile tilapia (Oreochromis niloticus) as well as some immune-related genes. At first, genomic ANF IV gene was characterized from one fish; 124 amino acids were identified with 92.7% similarity with that on the gene bank. Expression of ANF IV and immune-related genes were done twice, once at the end of December (winter sample, temperature 14 A degrees C) and the other at August (summer sample, temperature 36 A degrees C). Assessment of ANF IV gene expression in different organs of fish was done; splenic mRNA was used for assessment of immune-related gene transcripts (CXCl2 chemokine, cc-chemokine, INF-3A, and MHC II beta). Winter expression analysis of AFP IV in O. niloticus revealed significant upregulation of mRNA transcript levels in the intestine, gills, skin, spleen, liver, and brain with 324.03-, 170.06-, 107.63-, 97.61-, 94.35-, and 27.85-folds, respectively. Furthermore, upregulation in the gene was observed in some organs during summer: in the liver, gills, skin, intestine, and brain with lower levels compared with winter. The level of expression of immune-related genes in winter is significantly higher than summer in all assessed genes. Cc-chemokine gene expression was the most affected in both winter and summer. Variable expression profile of ANF IV in different organs and in different seasons together with its amino acid similarity of N-terminal and C-terminal with apolipoprotein (lipid binder) and form of high-density lipoprotein (HDL) suggests a different role for this protein which may be related to lipid metabolism.
引用
收藏
页码:515 / 525
页数:11
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