Pairing Bacteroides vulgatus LPS Structure with Its Immunomodulatory Effects on Human Cellular Models

被引：68

作者：

Di Lorenzo, Flaviana ^{[1
,8
]}

Pither, Molly D. ^{[1
]}

Martufi, Michela ^{[2
]}

Scarinci, Ilaria ^{[2
]}

Guzman-Caldentey, Joan ^{[3
]}

Lakomiec, Ewelina ^{[4
]}

Jachymek, Wojciech ^{[4
]}

Bruijns, Sven C. M. ^{[5
,6
]}

Martin Santamaria, Sonsoles ^{[3
]}

Frick, Julia-Stephanie ^{[7
]}

van Kooyk, Yvette ^{[5
,6
]}

Chiodo, Fabrizio ^{[5
,6
]}

Silipo, Alba ^{[1
,8
]}

Bernardini, Maria Lina ^{[2
]}

Molinaro, Antonio ^{[1
,8
]}

机构：

[1] Univ Naples Federico II, Dept Chem Sci, I-80126 Naples, Italy

[2] Sapienza Univ Rome, Dept Biol & Biotechnol C Darwin, I-00185 Rome, Italy

[3] CSIC, Dept Struct & Chem Biol, Ctr Invest Biol, Madrid 28040, Spain

[4] Polish Acad Sci, Hirszfeld Inst Immunol & Expt Therapy, PL-53114 Wroclaw, Poland

[5] Vrije Univ Amsterdam, Amsterdam UMC, Dept Mol Cell Biol & Immunol, Amsterdam Infect & Immun Inst, NL-1081 HV Amsterdam, Netherlands

[6] Vrije Univ Amsterdam, Amsterdam UMC, Canc Ctr Amsterdam, NL-1081 HV Amsterdam, Netherlands

[7] Univ Tubingen, Inst Med Microbiol & Hyg, D-72076 Tubingen, Germany

[8] Univ Naples Federico II, Task Force Microbiome Studies, I-80126 Naples, Italy

来源：

ACS CENTRAL SCIENCE | 2020年 / 6卷 / 09期

基金：

欧盟地平线“2020”;

关键词：

ABSOLUTE-CONFIGURATION; CLINICAL ISOLATE; LIPID-A; LIPOPOLYSACCHARIDE; BACTERIAL; RECOGNITION; ENDOTOXIN; PROTEIN; OLIGOSACCHARIDE; INTERFERENCE;

D O I：

10.1021/acscentsci.0c00791

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The gut microbiota guide the development of the host immune system by setting a systemic threshold for immune activation. Lipopolysaccharides (LPSs) from gut bacteria are able to trigger systemic and local proinflammatory and immunomodulatory responses, and this capability strongly relies on their fine structures. Up to now, only a few LPS structures from gut commensals have been elucidated; therefore, the molecular motifs that may be important for LPS-mammalian cell interactions at the gut level are still obscure. Here, we report on the full structure of the LPS isolated from one of the prominent species of the genus Bacteroides, Bacteroides vulgatus. The LPS turned out to consist of a particular chemical structure based on hypoacylated and mono-phosphorylated lipid A and with a galactofuranose-containing core oligosaccharide and an O-antigen built up of mannose and rhamnose. The evaluation of the immunological properties of this LPS on human in vitro models revealed a very interesting capability to produce anti-inflammatory cytokines and to induce a synergistic action of MD-2/TLR4- and TLR2-mediated signaling pathways.

引用

页码：1602 / 1616

页数：15

共 61 条

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