Elevated circulatory MMP-2 and MMP-9 levels and activities in patients with rheumatoid arthritis and systemic lupus erythematosus

被引:94
作者
Chang, Yih-Hsin [2 ]
Lin, I-Ling [3 ]
Tsay, Gregory J. [4 ,5 ]
Yang, Shun-Chun [2 ]
Yang, Tzi-Peng [2 ]
Ho, Kuo-Ting [6 ]
Hsu, Tsai-Ching [5 ]
Shiau, Ming-Yuh [1 ]
机构
[1] Hung Kuang Univ, Taichung, Taiwan
[2] Chung Shan Med Univ, Sch Med Lab & Biotechnol, Taichung, Taiwan
[3] Kaohsiung Med Univ, Fac Biomed, Lab Sci, Kaohsiung, Taiwan
[4] Chung Shan Med Univ Hosp, Dept Internal Med, Taichung, Taiwan
[5] Chung Shan Med Univ, Inst Immunol, Taichung, Taiwan
[6] Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan
关键词
matrix metalloproteinase-2; matrix metalloproteinase-9; rheumatoid arthritis; systemic lupus erythematosus;
D O I
10.1016/j.clinbiochem.2008.04.012
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objectives: Matrix metalloproteinases (MMPs) are suggested to play important roles in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). This study is to examine the MMPs expressions and activities in Taiwanese RA and SLE patients. Design and methods: Levels and activities of plasma MMP-2 and MMP-9 were investigated by enzyme-linked immunosorbent assay and zymography, respectively. Results: MMP-2 levels in control subjects, RA and SLE patients were 146.1 +/- 34.2, 194.0 +/- 24.2 and 208.9 +/- 75.9 ng/mL respectively, and for MMP-9 were 51.4 +/- 57.1, 567.7 +/- 313.1 and 208.7 +/- 105.5 ng/mL respectively. Both MMP-2 and MMP-9 levels and activities from all patients were significantly higher than that from control subjects. Conclusions: MMP-2 levels in both patients groups were approximately 1.3-1.4 folds higher than that in control subjects, notably, MMP-9 levels were 11- and 4-folds significantly higher, respectively, in RA and SLE patients. The results which MMP-2 and MMP-9 levels and activities are significantly elevated support the involvement of MMPs proteins in these autoimmune disorders. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:955 / 959
页数:5
相关论文
共 24 条
[1]   Expression of matrix metalloproteinase 9 (96-kd gelatinase B) in human rheumatoid arthritis [J].
Ahrens, D ;
Koch, AE ;
Pope, RM ;
SteinPicarella, M ;
Niedbala, MJ .
ARTHRITIS AND RHEUMATISM, 1996, 39 (09) :1576-1587
[2]   Increased serum matrix metalloproteinase 9 levels in systemic lupus erythematosus patients with neuropsychiatric manifestations and brain magnetic resonance imaging abnormalities [J].
Ainiala, H ;
Hietaharju, A ;
Dastidar, P ;
Loukkola, J ;
Lehtimäki, T ;
Peltola, J ;
Korpela, M ;
Heinonen, T ;
Nikkari, ST .
ARTHRITIS AND RHEUMATISM, 2004, 50 (03) :858-865
[3]  
ARNETT FC, 1987, ARTHRITIS RHEUM, V31, P315
[4]   Increased tissue inhibitor of metalloproteinase-1 expression and inhibition of gelatinase A activity in buccal mucosal fibroblasts by arecoline as possible mechanisms for oral submucous fibrosis [J].
Chang, YC ;
Yang, SF ;
Tai, KW ;
Chou, MY ;
Hsieh, YS .
ORAL ONCOLOGY, 2002, 38 (02) :195-200
[5]   Matrix metalloproteinase 2 may be a marker of microangiopathy in children and adolescents with type 1 diabetes [J].
Derosa, G ;
Avanzini, MA ;
Geroldi, D ;
Fogari, R ;
Lorini, R ;
De Silvestri, A ;
Tinelli, C ;
Rondini, G ;
D'Annunzio, G .
DIABETES CARE, 2004, 27 (01) :273-274
[6]   Activity of matrix metalloproteinase-9 is elevated in sera of patients with systemic lupus erythematosus [J].
Faber-Elmann, A ;
Sthoeger, Z ;
Tcherniack, A ;
Dayan, M ;
Mozes, E .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2002, 127 (02) :393-398
[7]   Markedly elevated serum MMP-9 (gelatinase B) levels in rheumatoid arthritis: A potentially useful laboratory marker [J].
Gruber, BL ;
Sorbi, D ;
French, DL ;
Marchese, RRJ ;
Nuovo, GJ ;
Kew, RR ;
Arbeit, LA .
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 78 (02) :161-171
[8]   The role of matrix metalloproteinase-2 and matrix metalloproteinase-9 in antibody-induced arthritis [J].
Itoh, T ;
Matsuda, H ;
Tanioka, M ;
Kuwabara, K ;
Itohara, S ;
Suzuki, R .
JOURNAL OF IMMUNOLOGY, 2002, 169 (05) :2643-2647
[9]  
Iwaki-Egawa S, 2001, J RHEUMATOL, V28, P485
[10]   QUANTITATIVE ZYMOGRAPHY - DETECTION OF PICOGRAM QUANTITIES OF GELATINASES [J].
KLEINER, DE ;
STETLERSTEVENSON, WG .
ANALYTICAL BIOCHEMISTRY, 1994, 218 (02) :325-329