Nano-TiO2 enhances biofilm formation in a bacterial isolate from activated sludge of a waste water treatment plant

被引：17

作者：

Mathur, Ankita ^{[1
]}

Parashar, Abhinav ^{[1
]}

Chandrasekaran, N. ^{[1
]}

Mukherjee, Amitava ^{[1
]}

机构：

[1] VIT Univ, Ctr Nanobiotechnol, Vellore 632014, Tamil Nadu, India

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2017年 / 116卷

关键词：

TiO2 NPs (Titanium dioxide nanoparticles); Irradiation condition; Anaerobic; Bacterial isolate; EPS; Biofilm; EXTRACELLULAR POLYMERIC SUBSTANCES; METAL-OXIDE NANOPARTICLES; SURFACE-AREA; TOXICITY; EPS; FLOCCULATION; TIO2;

D O I：

10.1016/j.ibiod.2016.09.024

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

TiO2 NPs is one of the major components of paints and sunscreens and also acts as a catalyst for waste water treatment. The study demonstrates the toxic effects of TiO2 NPs on an anaerobic bacterium, Macrococcus caseolyticus, isolated from the activated sludge of a waste water treatment facility. The cytotoxicity assessment under UVA, visible light, and dark conditions revealed a dose- and exposure dependent reduction in viability. The examination of cytotoxicity was performed with membrane permeability assessment through LDH (Lactate dehydrogenase) detection. The formation of exopolymeric substances (EPS) was observed to be dose-dependent, and maximum EPS release was noted under UVA condition. The scanning electron microscopy described the aggregation tendency of biofilm to be more pronounced under UVA condition when treated with TiO2 NPs as compared to the control biofilm. Uptake of TiO2 NPs by the biofilm indicated possible remediation of the NPs in waste water by these organisms. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：17 / 25

页数：9