An orthogonal DNA replication system in yeast

被引：1

作者：

Ravikumar, Arjun ^{[1
]}

Arrieta, Adrian ^{[1
]}

Liu, Chang C. ^{[1
,2
,3
]}

机构：

[1] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Chem, Irvine, CA 92717 USA

[3] Univ Calif Irvine, Ctr Complex Biol Syst, Irvine, CA USA

来源：

NATURE CHEMICAL BIOLOGY | 2014年 / 10卷 / 03期

基金：

美国国家科学基金会;

关键词：

SACCHAROMYCES-CEREVISIAE; KLUYVEROMYCES-LACTIS; SPONTANEOUS MUTATION; KILLER PLASMID; DECARBOXYLASE; EVOLUTION; MICROBES; FUSION; PGKL1;

D O I：

10.1038/NCHEMBIO.1439

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An extranuclear replication system, consisting of an orthogonal DNA plasmid-DNA polymerase pair, was developed in Saccharomyces cerevisiae. Engineered error-prone DNA polymerases showed complete mutational targeting in vivo: per-base mutation rates on the plasmid were increased substantially and remained stable with no increase in genomic rates. Orthogonal replication serves as a platform for in vivo continuous evolution and as a system whose replicative properties can be manipulated independently of the host's.

引用

页码：175 / +

页数：6

共 23 条

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