Three-dimensional cellular-level imaging using full-field optical coherence tomography

被引：109
作者：
Dubois, A [1 ]
Moneron, G [1 ]
Grieve, K [1 ]
Boccara, AC [1 ]
机构：
[1] CNRS, UPR A0005, Lab Opt Phys, Ecole Super Phys & Chim Ind, F-75231 Paris 5, France
来源：
PHYSICS IN MEDICINE AND BIOLOGY | 2004年 / 49卷 / 07期
关键词：
D O I：
10.1088/0031-9155/49/7/010
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
An ultrahigh-resolution full-field optical coherence tomography (OCT) system has been developed for cellular-level imaging of biological media. The system is based on a Linnik interference microscope illuminated with a tungsten halogen lamp, associated with a high-resolution CCD camera. En face tomographic images are produced in real time, with the best spatial resolution ever achieved in OCT (0.7 mum x 0.9 mum, axial x transverse). A shot-noise limited detection sensitivity of 80 dB can be reached with an acquisition time per image of 1 s. Images of animal ophthalmic biopsies and vegetal tissues are shown.
引用
收藏
页码：1227 / 1234
页数：8
相关论文
共 20 条
  • [1] In vivo cellular optical coherence tomography imaging
    Boppart, SA
    Bouma, BE
    Pitris, C
    Southern, JF
    Brezinski, ME
    Fujimoto, JG
    [J]. NATURE MEDICINE, 1998, 4 (07) : 861 - 865
  • [2] HIGH-RESOLUTION OPTICAL COHERENCE TOMOGRAPHIC IMAGING USING A MODE-LOCKED TI-AL2O3 LASER SOURCE
    BOUMA, B
    TEARNEY, GJ
    BOPPART, SA
    HEE, MR
    BREZINSKI, ME
    FUJIMOTO, JG
    [J]. OPTICS LETTERS, 1995, 20 (13) : 1486 - 1488
  • [3] Self-phase-modulated Kerr-lens mode-locked Cr:forsterite laser source for optical coherence tomography
    Bouma, BE
    Tearney, GJ
    Bilinsky, IP
    Golubovic, B
    Fujimoto, JG
    [J]. OPTICS LETTERS, 1996, 21 (22) : 1839 - 1841
  • [4] In vivo ultrahigh-resolution optical coherence tomography
    Drexler, W
    Morgner, U
    Kärtner, FX
    Pitris, C
    Boppart, SA
    Li, XD
    Ippen, EP
    Fujimoto, JG
    [J]. OPTICS LETTERS, 1999, 24 (17) : 1221 - 1223
  • [5] Ultrahigh-resolution optical coherence tomography
    Drexler, W
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 2004, 9 (01) : 47 - 74
  • [6] High-resolution full-field optical coherence tomography with a Linnik microscope
    Dubois, A
    Vabre, L
    Boccara, AC
    Beaurepaire, E
    [J]. APPLIED OPTICS, 2002, 41 (04) : 805 - 812
  • [7] Fercher A F, 1996, J Biomed Opt, V1, P157, DOI 10.1117/12.231361
  • [8] Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography
    Gloesmann, M
    Hermann, B
    Schubert, C
    Sattmann, H
    Ahnelt, PK
    Drexler, W
    [J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2003, 44 (04) : 1696 - 1703
  • [9] Dispersion effects in partial coherence interferometry: Implications for intraocular ranging
    Hitzenberger, CK
    Baumgartner, A
    Drexler, W
    Fercher, AF
    [J]. JOURNAL OF BIOMEDICAL OPTICS, 1999, 4 (01) : 144 - 151
  • [10] Three-dimensional imaging of the human retina by high-speed optical coherence tomography
    Hitzenberger, CK
    Trost, P
    Lo, PW
    Zhou, QY
    [J]. OPTICS EXPRESS, 2003, 11 (21): : 2753 - 2761
12