Giardia duodenalis: Effects of an ozonized sunflower oil product (Oleozon®) on in vitro trophozoites

被引：18

作者：

Hernandez, Frank ^{[1
]}

Hernandez, Damian ^{[1
]}

Zamora, Zullyt ^{[1
]}

Diaz, Maritza ^{[1
]}

Ancheta, Odelsa ^{[1
,2
]}

Rodriguez, Sandra ^{[1
,2
]}

Torres, Dinorah ^{[1
,3
]}

机构：

[1] Natl Ctr Sci Res, Ozone Res Ctr, Dept Biomed, Havana 16000, Cuba

[2] Natl Ctr Sci Res, Lab Elect Microscopy, Biotechnol Unit, Havana 16000, Cuba

[3] Pedro Kouri Inst, Dept Parasitol, Havana, Cuba

来源：

EXPERIMENTAL PARASITOLOGY | 2009年 / 121卷 / 03期

关键词：

Ozonized sunflower oil; Ozone therapy; Giardiasis; Giardia duodenalis; Oleozon; SPECTROSCOPIC CHARACTERIZATION; LAMBLIA;

D O I：

10.1016/j.exppara.2008.10.009

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The ozonized sunflower oil product (Oleozon (R)) was investigated to explore its cytotoxic activity on Giardia duodenalis in vitro cultivated trophozites. Oleozon (R) produced inactivation of Giardia trophozoites in a dose- and cell density-dependent manner. Thirty microliter of Oleozon (R) with peroxide index value of 500 equivalent-mmol of activated oxygen per kilogram were used to achieve a 100% inhibition (<-4.00 log unit) of trophozoites from an initial inoculum of 15 x 104 cells. This potent effect was confirmed by transmission electron microscopy where morphological deterioration of superficial structures mainly in the ventral disc, and formation of a great number of micro vesicles in the cytoplasm were found. We concluded that a direct chemical-oxidation attack by the active substances from Oleozon (R) is one of the causes of the parasitocidal effect of this product. We suggest that the dose and cell density-de pendent effect must be taken into account when prescription of this product for giardiasis treatment in humans. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：208 / 212

页数：5