A study on the interaction between 3-spiro-piperidones and bovine serum albumin using spectroscopic approaches

被引：2

作者：

Yu, Xianyong ^{[1
]}

Yao, Qing ^{[1
]}

Tao, Hongwen ^{[1
]}

Yang, Ying ^{[1
]}

Li, Lei ^{[1
]}

Zheng, Baishu ^{[1
]}

Zhu, Shizhong ^{[2
]}

机构：

[1] Hunan Univ Sci & Technol, Key Lab Theoret Chem & Mol Simulat, Key Lab QSAR QSPR, Sch Chem & Chem Engn,Hunan Prov Coll,Minist Educ, Xiangtan 411201, Peoples R China

[2] Farming Dev Serv Ctr, Tianjin 300016, Peoples R China

来源：

LUMINESCENCE | 2013年 / 28卷 / 05期

基金：

中国国家自然科学基金;

关键词：

bovine serum albumin; 3-spiro-3-piperidine-2; 3-dione; interaction; fluorescence spectroscopy; ultraviolet spectroscopy; BINDING;

D O I：

10.1002/bio.2420

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The interaction between 3-spiro-2-pyrrolidine-3-spiro-3-piperidine-2,3-dione (PPD) and bovine serum albumin (BSA) in aqueous solution was studied using fluorescence and UV-vis spectroscopy. Fluorescence emission data revealed that BSA (1.00x10(-5)mol/L) fluorescence was statically quenched by PPD at various concentrations, which implies that a PPD-BSA complex was formed. The binding constant (K-A), the number of binding sites (n) and the specific binding site of the PPD with BSA were determined. Energy-transfer efficiency parameters were determined and the mechanism of the interaction discussed. The thermodynamic parameters, G, H and S, were obtained according to van't Hoff's equation, showing the involvement of hydrophobic forces in these interactions. The effect of PPD acting on the BSA conformation was detected by synchronous fluorescence. Copyright (c) 2012 John Wiley & Sons, Ltd.

引用

页码：705 / 712

页数：8

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