Detection of digoxin in urine samples by surface-assisted laser desorption/ionization mass spectrometry with dispersive liquid-liquid microextraction

被引：26

作者：

Cheng, Mei-Ching ^{[1
]}

Chi, Kai-Ming ^{[2
]}

Chang, Sarah Y. ^{[1
]}

机构：

[1] Natl Kaohsiung Normal Univ, Dept Chem, Kaohsiung 824, Taiwan

[2] Natl Chung Cheng Univ, Dept Chem & Biochem, Chiayi 621, Taiwan

来源：

TALANTA | 2013年 / 115卷

关键词：

Surface-assisted laser desorption/ionization; mass spectrometry; Dispersive liquid-liquid microextraction; Digoxin; Urine sample; Colloidal palladium; HUMAN PLASMA; NANOPARTICLE MATRICES; CARDIAC-GLYCOSIDES; SMALL MOLECULES; CHROMATOGRAPHY; QUANTIFICATION; QUANTITATION; VALIDATION; MS;

D O I：

10.1016/j.talanta.2013.04.040

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method for the detection of digoxin using dispersive liquid-liquid microextraction (DLLME) coupled to the surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. Acetone and chloroform were used as the disperser solvent and extraction solvents, respectively. After the extraction, digoxin was detected using SALDI/MS with colloidal palladium as the matrix. Under optimal extraction and detection conditions, the calibration curve, which ranged from 0.01 to 0.50 mu M, was observed to be linear. The limit of detection (LOD) at a signal-to-noise ratio of 3 was 2 nM for digoxin. With a sample-to-extract volume ratio of 400, the enrichment factor for digoxin was calculated to be 252. This novel method was successfully applied for the determination of digoxin in human urine samples. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：123 / 128

页数：6

共 29 条

[1]

[Anonymous], 2001, Guidance for industry, bioanalytical method validation

[2] Fluorescence detection of cardenolides in reversed-phase high-performance liquid chromatography after post-column derivatization [J].

Belsner, K ;

Buchele, B .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 682 (01) :95-107

[3] Synthesis, characterization and application of modified Pd nanoparticles as preconcentration probes for selective enrichment/analysis of proteins via hydrophobic interactions from real-world samples using nanoparticle-liquid-liquid microextraction coupled to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [J].

Bhat, Abdul R. ;

Wu, Hui-Fen .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2010, 24 (24) :3547-3552

[4] Validation of method for determination of different classes of pesticides in aqueous samples by dispersive liquid-liquid microextraction with liquid chromatography-tandem mass spectrometric detection [J].

Caldas, Sergiane Souza ;

Costa, Fabiane Pinho ;

Primel, Ednei Gilberto .

ANALYTICA CHIMICA ACTA, 2010, 665 (01) :55-62

[5] Determination of Macrolide Antibiotics Using Dispersive Liquid-Liquid Microextraction Followed by Surface-Assisted Laser Desorption/Ionization Mass Spectrometry [J].

Chen, Kuan-Yu ;

Yang, Thomas C. ;

Chang, Sarah Y. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2012, 23 (06) :1157-1160

[6] Determining estrogens using surface-assisted laser desorption/ionization mass spectrometry with silver nanoparticles as the matrix [J].

Chiu, Tai-Chia ;

Chang, Lin-Chau ;

Chiang, Cheng-Kang ;

Chang, Huan-Tsung .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (09) :1343-1346

[7] Affinity surface-assisted laser desorption/ionization mass spectrometry for peptide enrichment [J].

Coffinier, Yannick ;

Nguyen, Nhung ;

Drobecq, Herve ;

Melnyk, Oleg ;

Thomy, Vincent ;

Boukherroub, Rabah .

ANALYST, 2012, 137 (23) :5527-5532

[8] Diamond nanowires for highly sensitive matrix-free mass spectrometry analysis of small molecules [J].

Coffinier, Yannick ;

Szunerits, Sabine ;

Drobecq, Herve ;

Melnyk, Oleg ;

Boukherroub, Rabah .

NANOSCALE, 2012, 4 (01) :231-238

[9] INACCURACIES IN DIGOXIN MEASUREMENT [J].

COOK, JD ;

KOCH, TR ;

COOK, MS ;

KNOBLOCK, EC .

CLINICAL BIOCHEMISTRY, 1988, 21 (06) :353-357

[10] ULTRAVIOLET VISIBLE ABSORPTION-SPECTRA OF THE COLLOIDAL METALLIC ELEMENTS [J].

CREIGHTON, JA ;

EADON, DG .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1991, 87 (24) :3881-3891

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