Refining the description of protein chromatography

被引：14

作者：

Lightfoot, EN ^{[1
]}

Coffman, JL ^{[1
]}

Lode, F ^{[1
]}

Yuan, QS ^{[1
]}

Perkins, TW ^{[1
]}

Root, TW ^{[1
]}

机构：

[1] BIOSEPRA INC,MARLBOROUGH,MA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 760卷 / 01期

关键词：

stationary phases; LC; column performance; proteins;

D O I：

10.1016/S0021-9673(96)00843-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Review of design procedures used for protein chromatography shows the need for considerable refinement to meet the demands of high flow-rates and complex processes characteristic of modern commercial operation. Suggestions are made for dealing with the skewness of effluent curves from differential chromatography in interpreting such data and also for scaling up laboratory processes. Proton-specific nuclear magnetic resonance images of small commercial columns show significant non-uniformity of packing density, and suggestions are made for a study of the effects of column shape, size and packing procedures on column efficiency.

引用

页码：139 / 149

页数：11

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