Expression of pannexin family of proteins in the retina

被引：92

作者：

Dvoriantchikova, G

Ivanov, D

Panchin, Y

Shestopalov, VI

机构：

[1] Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Dept Ophthalmol, Miami, FL 33136 USA

[2] RAS, NI Vavilov Gen Genet Res Inst, Moscow 117901, Russia

[3] RAS, Inst Informat Transmiss Problems, Moscow 117901, Russia

[4] Moscow MV Lomonosov State Univ, AN Belozersky Inst, Moscow, Russia

[5] Univ Miami, Sch Med, Dept Cell Biol & Anat, Miami, FL 33152 USA

来源：

FEBS LETTERS | 2006年 / 580卷 / 09期

关键词：

pannexin farnily; gap junction proteins; retina; retinal ganglion cells; gene expression; antibody;

D O I：

10.1016/j.febslet.2006.03.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of the Panx1 and Panx2 members of the pannexin family of gap junction proteins was studied in the retina by in situ hybridization and qRT-PCR. Both pannexins showed robust expression across the retina with predominant accumulation in the retinal ganglion cells (RGCs). In concordance, immuno-histochemical analysis showed accumulation of the Panx1 protein in RGCs, amacrine, horizontal cells and their processes. Two Panx1 isoforms were detected: a ubiquitously expressed 58 kDa protein, and a 43 kDa isoform that specifically accumulated in the retina and brain. Our results indicated that Panx1 and Panx2 are abundantly expressed in the retina, and may therefore contribute to the electrical and metabolic coupling, or to signaling between retinal neurons via the secondary messengers. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2178 / 2182

页数：5

共 23 条

[11] Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium [J].