Whole transcriptome analysis of the pectoralis major muscle reveals molecular mechanisms involved with white striping in broiler chickens

被引:48
作者
Marchesi, J. A. P. [1 ,2 ,3 ]
Ibelli, A. M. G. [1 ,2 ,3 ]
Peixoto, J. O. [1 ,2 ]
Cantao, M. E. [1 ,2 ]
Pandolfi, J. R. C. [1 ,2 ]
Marciano, C. M. M. [4 ]
Zanella, R. [7 ]
Settles, M. L. [5 ]
Coutinho, L. L. [6 ]
Ledur, M. C. [1 ,2 ,4 ]
机构
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP, Brazil
[2] Embrapa Suinos & Aves, BR-89715899 Concordia, SC, Brazil
[3] Univ Contestado, BR-89711330 Concordia, SC, Brazil
[4] Univ Estado Santa Catarina, UDESC Oeste, Programa Posgrad Zootecnia, BR-89815630 Chapeco, SC, Brazil
[5] Univ Calif Davis, UC Davis Genome Ctr, Davis, CA 95616 USA
[6] Univ Sao Paulo, Dept Anim Sci, BR-13418900 Piracicaba, SP, Brazil
[7] Univ Passo Fundo, BR-9905290 Passo Fundo, RS, Brazil
关键词
muscle disorder; breast myopathy; RNA-Seq; SKELETAL-MUSCLE; WOODEN BREAST; MEAT QUALITY; GROWTH; MYOPATHIES; GENES; CELLS; REGENERATION; DEFICIENCY; PARAMETERS;
D O I
10.3382/ps/pey429
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
White striping (WS) is one of the most common myopathies identified in broiler chickens leading to substantial production losses, where the incidence reaches 12% in commercial chickens. It occurs primarily in heavier chickens being a modification of the breast muscle characterized by the presence of pale parallel streaks in the same orientation of the muscle fibers. Since the WS etiology remains unclear, we aimed to identify the biological and genetic mechanisms involved in its occurrence through the whole transcriptome analysis of WS in affected and unaffected chicken breast muscles. A total of 11,177 genes were expressed in the pectoralis major muscle. Out of those, 1,441 genes were differentially expressed (FDR <= 0.01) between the two analyzed groups, being, respectively, 772 genes upregulated and 669 downregulated in the WS affected group. A total of 36 significantly overrepresented GO terms related to WS myopathy were enriched, and the most relevant biological processes were activation of immune system, angiogenesis, hypoxia, cell death, and striated muscle contraction. The unbalance of those biological processes may trigger the occurrence of the WS phenotype in broilers. The possible lack of capillary blood supply homogeneously in the muscle triggers the hypoxia, following the activation of glycolysis, calcium signaling and apoptosis related genes facilitating the tissue damage and WS incidence.
引用
收藏
页码:590 / 601
页数:12
相关论文
共 72 条
[1]   Oxidative Stress and Metabolic Perturbations in Wooden Breast Disorder in Chickens [J].
Abasht, Behnam ;
Mutryn, Marie F. ;
Michalek, Ryan D. ;
Lee, William R. .
PLOS ONE, 2016, 11 (04)
[2]   Genetic parameters of white striping in relation to body weight, carcass composition, and meat quality traits in two broiler lines divergently selected for the ultimate pH of the pectoralis major muscle [J].
Alnahhas, Nabeel ;
Berri, Cecile ;
Chabault, Marie ;
Chartrin, Pascal ;
Boulay, Maryse ;
Bourin, Marie Christine ;
Le Bihan-Duval, Elisabeth .
BMC GENETICS, 2016, 17
[3]   HTSeq-a Python']Python framework to work with high-throughput sequencing data [J].
Anders, Simon ;
Pyl, Paul Theodor ;
Huber, Wolfgang .
BIOINFORMATICS, 2015, 31 (02) :166-169
[4]  
[Anonymous], 2015, OECD Companion to the Inventory of Support Measures for Fossil Fuels 2015, P41, DOI DOI 10.1787/AGROUTLOOK-2015-EN
[5]   The genetic basis of pectoralis major myopathies in modern broiler chicken lines [J].
Bailey, Richard A. ;
Watson, Kellie A. ;
Bilgili, S. F. ;
Avendano, Santiago .
POULTRY SCIENCE, 2015, 94 (12) :2870-2879
[6]  
Basic Ivan, 1997, Periodicum Biologorum, V99, P313
[7]   CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].
BENJAMINI, Y ;
HOCHBERG, Y .
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300
[8]   Unraveling the cause of white striping in broilers using metabolomics [J].
Boerboom, Gavin ;
van Kempen, Theo ;
Navarro-Villa, Alberto ;
Perez-Bonilla, Adriano .
POULTRY SCIENCE, 2018, 97 (11) :3977-3986
[9]   Increased Wnt signaling during aging alters muscle stem cell fate and increases fibrosis [J].
Brack, Andrew S. ;
Conboy, Michael J. ;
Roy, Sudeep ;
Lee, Mark ;
Kuo, Calvin J. ;
Keller, Charles ;
Rando, Thomas A. .
SCIENCE, 2007, 317 (5839) :807-810
[10]   BLAST plus : architecture and applications [J].
Camacho, Christiam ;
Coulouris, George ;
Avagyan, Vahram ;
Ma, Ning ;
Papadopoulos, Jason ;
Bealer, Kevin ;
Madden, Thomas L. .
BMC BIOINFORMATICS, 2009, 10