Hybrid monolithic columns coated with cellulose tris(3,5-dimethylphenyl-carbamate) for enantioseparations in capillary electrochromatography and capillary liquid chromatography

被引:31
作者
Ou, Junjie [1 ]
Lin, Hui [1 ,2 ]
Tang, Shouwan [3 ]
Zhang, Zhenbin [1 ,2 ]
Dong, Jing [1 ]
Zou, Hanfa [1 ]
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
[3] Taizhou Coll, Linhai 317000, Peoples R China
关键词
Hybrid monolithic column; Chiral stationary phase; Capillary electrochromatography; Capillary liquid chromatography; Substituted cellulose derivatives; Enantioseparation; CHIRAL STATIONARY PHASES; ELECTROMIGRATION TECHNIQUES; POLYSACCHARIDE DERIVATIVES; ENANTIOMER SEPARATION; SILICA; CEC; POLYMER; FABRICATION; TRENDS; HPLC;
D O I
10.1016/j.chroma.2012.09.022
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A hybrid monolithic capillary column synthesized with (3-chloropropyl)-trimethoxysilane (CPTMS) and tetramethoxysilane (TMOS) via sol-gel chemistry was in situ coated with cellulose tris(3,5-dimethylphenyl-carbamate) (CDMPC) for enantioseparations in capillary electrochromatography (CEC) and capillary liquid chromatography (CLC). Prior to coating, the prepared CP-silica hybrid monolith was straightforwardly modified with diethylenetriamine (DETA) to introduce -NH2 functionalities via the nucleophilic substitution reaction, which generate the stronger EOF for CEC. The coating condition was optimized to obtain a stable and reproducible chiral stationary phase for enantioseparation. The results indicated that racemic benzoin was baseline separated on the resulting hybrid monolith coated with 30 mg/mL CDMPC in CEC, while several racemates were successfully enantioseparated on the resulting CP-silica hybrid monolithic column coated with 60 mg/mL CDMPC in CLC with RP and NP modes. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:372 / 378
页数:7
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