Metabonomics Study of Essential Hypertension and Its Chinese Medicine Subtypes by Using Gas Chromatography-Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy

被引：20

作者：

Li, Yunlun ^{[1
]}

Nie, Lei ^{[2
]}

Jiang, Haiqiang ^{[3
]}

Lin, Jiamao ^{[3
]}

Zhou, Honglei ^{[3
]}

Xie, Jun ^{[3
]}

Qu, Zhengjun ^{[3
]}

Qi, Dongmei ^{[3
]}

Zhang, Yunhui ^{[1
]}

机构：

[1] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Dept Cardiol, Jinan 250011, Peoples R China

[2] Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Peoples R China

[3] Shandong Univ Tradit Chinese Med, Expt Ctr, Jinan 250355, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 2013卷

基金：

中国国家自然科学基金;

关键词：

LIQUID-CHROMATOGRAPHY; INSULIN-RESISTANCE; SYSTEMS BIOLOGY; RAT MODEL; PERFORMANCE; PLASMA;

D O I：

10.1155/2013/625906

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

A metabonomic study was performed to investigate the metabolic mechanism of essential hypertension and its Chinese medicine subtypes, including "Yin-deficiency and Yang-hyperactivity syndrome" (YDYHS) and "Yin-Yang deficiency syndrome" (YYDS). Plasma samples from 22 healthy volunteers, 31 hypertensive patients with YDYHS, and 29 hypertensive patients with YYDS were analyzed by H-1-NMR spectroscopy and gas chromatography coupled with mass spectrometry (GC-MS). The three groups were distinctly classified by principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA). According to identified biomarkers and their related pathways, abnormal glucose metabolism might be the main common pathway from YDYHS to YYDS, and sympathetic nervous system activation would play an important role in the pathogenesis of YDYHS, while a low metabolic rate usually occurred in YYDS.

引用

页数：9

共 34 条

[21] Adoption in China of Clinical Practice Guidelines for Hypertension Using Traditional Chinese Medical Approaches: A Literature Review Based on Clinical Studies [J].

Shi, Nannan ;

Han, Xuejie ;

Yu, Wenya ;

Wang, Liying ;

Lu, Aiping .

JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE, 2013, 19 (01) :1-8

[22] Comparative Analysis of Novel Noninvasive Renal Biomarkers and Metabonomic Changes in a Rat Model of Gentamicin Nephrotoxicity [J].

Sieber, Max ;

Hoffmann, Dana ;

Adler, Melanie ;

Vaidya, Vishal S. ;

Clement, Matthew ;

Bonventre, Joseph V. ;

Zidek, Nadine ;

Rached, Eva ;

Amberg, Alexander ;

Callanan, John J. ;

Dekant, Wolfgang ;

Mally, Angela .

TOXICOLOGICAL SCIENCES, 2009, 109 (02) :336-349

[23]

State Food and Drug Administration, 2002, GUID CLIN RES CHIN M

[24]

Sun G. R., 2006, BASIC THEORY TRADITI

[25]

Tang HR, 2006, PROG BIOCHEM BIOPHYS, V33, P401

[26]

Wang H., 2004, BIOCHEMISTRY

[27] Influence of insulin, sympathetic nervous system activity, and obesity on blood pressure: The Normative Aging Study [J].

Ward, KD ;

Sparrow, D ;

Landsberg, L ;

Young, JB ;

Vokonas, PS ;

Weiss, ST .

JOURNAL OF HYPERTENSION, 1996, 14 (03) :301-308

[28] NADPH oxidase contributes to vascular inflammation, insulin resistance, and remodeling in the transgenic (mRen2) rat [J].

Wei, Yongzhong ;

Whaley-Connell, Adam T. ;

Chen, Kemin ;

Habibi, Javad ;

Uptergrove, Grace M. -E. ;

Clark, Suzanne E. ;

Stump, Craig S. ;

Ferrario, Carlos M. ;

Sowers, James R. .

HYPERTENSION, 2007, 50 (02) :384-391

[29] Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models [J].

Wiklund, Susanne ;

Johansson, Erik ;

Sjostrom, Lina ;

Mellerowicz, Ewa J. ;

Edlund, Ulf ;

Shockcor, John P. ;

Gottfries, Johan ;

Moritz, Thomas ;

Trygg, Johan .

ANALYTICAL CHEMISTRY, 2008, 80 (01) :115-122

[30] A multi-analytical platform approach to the metabonomic analysis of plasma from normal and zucker (fa/fa) obese rats [J].

Williams, R ;

Lenz, EM ;

Wilson, AJ ;

Granger, J ;

Wilson, ID ;

Major, H ;

Stumpf, C ;

Plumb, R .

MOLECULAR BIOSYSTEMS, 2006, 2 (3-4) :174-183

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