Prediction and Identification of B-Cell Epitopes on Goose Parvovirus Capsid Protein

被引：0
作者：
Yu, Tian-fei [1 ,3 ]
Li, Ming [2 ]
Guo, Yuan [2 ]
Song, Guang-jun [2 ]
Li, Rong-xiu [3 ]
Xu, Ai-zhang [3 ]
Shao, Shu-li [1 ]
机构：
[1] Qiqihar Univ, Coll Life Sci & Agr Forestry, 42 Wenhua St, Qiqihar 161006, Peoples R China
[2] Qiqihar Univ, Coll Comp & Control Engn, Qiqihar 161006, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Life Sci Biotechnol, Key Lab, Minist Educ Microbial Metab, Shanghai 200240, Peoples R China
来源：
2011 INTERNATIONAL CONFERENCE ON ENVIRONMENT SCIENCE AND BIOTECHNOLOGY (ICESB 2011) | 2011年 / 8卷
关键词：
goose parvovirus; capsid protein; prediction; identification; epitope; LINKED-IMMUNOSORBENT-ASSAY; VIRAL DISEASE; VIRUS; GOSLINGS; GEESE;
D O I：
10.1016/j.proenv.2011.10.045
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The capsid protein (VP) gene of goose parvovirus (GPV) was translated into amino sequence by Editseq. The secondary structure and B-cell epitopes of the deduced protein were predicted by Protean. Combining the results according to these methods, the VP had complicated secondary structure. There were several potential B-cell epitopes on VP. By means of overlapping peptides expressed in Escherichia coli in combination with Western blot, B-cell epitopes were identified on VP. The results showed virtual linear immunodominant epitopes, which were found in seven fragments covering amino acid residues 35-81, 111-161, 423-453, 462-491, 531-577, 616-669, 678-732. (C) 2011 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of the Asia-Pacific Chemical, Biological & Environmental Engineering Society (APCBEES)
引用
收藏
页码：284 / 288
页数：5
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