Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

被引:34
作者
Al Khateeb, Wesam
Bahar, Eman
Lahham, Jamil
Schroeder, Dana
Hussein, Emad
机构
[1] Department of Biological Sciences, Yarmouk University, Irbid
[2] Department of Biological Sciences, University of Manitoba, Winnipeg
关键词
INDOLE-3-BUTYRIC ACID; RAPD; MICROPROPAGATION; STABILITY; ESTABLISHMENT; POPULATIONS; L;
D O I
10.1007/s12298-012-0149-z
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Moringa peregrina is an endangered species of Moringaceae. <Emphasis Type="ItalicUnderline" M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100 % shoot proliferation rate was observed on MS medium supplemented with 1.0 mg/l BA. On the other hand, MS medium supplemented with 1 mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation.
引用
收藏
页码:157 / 164
页数:8
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