Attomolar Label-Free Detection of DNA Hybridization with Electrolyte-Gated Graphene Field-Effect Transistors

被引：164

作者：

Campos, Rui ^{[1
,4
]}

Borme, Jerome ^{[1
]}

Guerreiro, Joana Rafaela ^{[2
]}

Machado, George, Jr. ^{[1
,3
]}

Cerqueira, Maria Fatima ^{[1
,3
]}

Petrovykh, Dmitri Y. ^{[2
]}

Alpuim, Pedro ^{[1
,3
]}

机构：

[1] Int Iberian Nanotechnol Lab, Dept Quantum & Energy Mat, P-4715330 Braga, Portugal

[2] Int Iberian Nanotechnol Lab, Dept Life Sci, P-4715330 Braga, Portugal

[3] Univ Minho, Ctr Phys, P-4710057 Braga, Portugal

[4] AXES Res Grp, Chem Dept, Groenenborgerlaan 171, B-2020 Antwerp, Belgium

来源：

ACS SENSORS | 2019年 / 4卷 / 02期

关键词：

biosensor; graphene; DNA; EGFET (electrolyte-gated field-effect transistor); planar technology; receded gate transistor; surface functionalization; ELECTRICAL DETECTION; GOLD; ALKANETHIOLS; BINDING; AMPLIFICATION; FABRICATION; MONOLAYERS; BIOSENSORS; NANOTUBES; SENSORS;

D O I：

10.1021/acssensors.8b00344

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this work, we develop a field-effect transistor with a two-dimensional channel made of a single graphene layer to achieve label-free detection of DNA hybridization down to attomolar concentration, while being able to discriminate a single nucleotide polymorphism (SNP). The SNP-level target specificity is achieved by immobilization of probe DNA on the graphene surface through a pyrene-derivative heterobifunctional linker. Biorecognition events result in a positive gate voltage shift of the graphene charge neutrality point. The graphene transistor biosensor displays a sensitivity of 24 mV/dec with a detection limit of 25 aM: the lowest target DNA concentration for which the sensor can discriminate between a perfect-match-target sequence and SNP-containing one.

引用

页码：286 / 293

页数：15

共 51 条

[41] ISOELECTRIC CHARACTERISTICS AND THE SECONDARY STRUCTURE OF SOME NUCLEIC-ACIDS [J].

SHERBET, GV ;

LAKSHMI, MS ;

CAJONE, F .

BIOPHYSICS OF STRUCTURE AND MECHANISM, 1983, 10 (03) :121-128

[42] Single-walled carbon nanotube chemoresistive label-free immunosensor for salivary stress biomarkers [J].

Tlili, Chaker ;

Cella, Lakshmi N. ;

Myung, Nosang V. ;

Shetty, Vivek ;

Mulchandani, Ashok .

ANALYST, 2010, 135 (10) :2637-2642

[43] Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives [J].

Veigas, Bruno ;

Fortunato, Elvira ;

Baptista, Pedro V. .

SENSORS, 2015, 15 (05) :10380-10398

[44] Graphene field-effect transistor array with integrated electrolytic gates scaled to 200 mm [J].

Vieira, N. C. S. ;

Borme, J. ;

Machado, G., Jr. ;

Cerqueira, F. ;

Freitas, P. P. ;

Zucolotto, V. ;

Peres, N. M. R. ;

Alpuim, P. .

JOURNAL OF PHYSICS-CONDENSED MATTER, 2016, 28 (08)

[45] Electrophoretic and field-effect graphene for all-electrical DNA array technology [J].

Xu, Guangyu ;

Abbott, Jeffrey ;

Qin, Ling ;

Yeung, Kitty Y. M. ;

Song, Yi ;

Yoon, Hosang ;

Kong, Jing ;

Ham, Donhee .

NATURE COMMUNICATIONS, 2014, 5

[46] Ultrasensitive label-free detection of DNA hybridization by sapphire-based graphene field-effect transistor biosensor [J].

Xu, Shicai ;

Jiang, Shouzhen ;

Zhang, Chao ;

Yue, Weiwei ;

Zou, Yan ;

Wang, Guiying ;

Liu, Huilan ;

Zhang, Xiumei ;

Li, Mingzhen ;

Zhu, Zhanshou ;

Wang, Jihua .

APPLIED SURFACE SCIENCE, 2018, 427 :1114-1119

[47] Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor [J].

Xu, Shicai ;

Zhan, Jian ;

Man, Baoyuan ;

Jiang, Shouzhen ;

Yue, Weiwei ;

Gao, Shoubao ;

Guo, Chengang ;

Liu, Hanping ;

Li, Zhenhua ;

Wang, Jihua ;

Zhou, Yaoqi .

NATURE COMMUNICATIONS, 2017, 8

[48] Carbon Nanomaterials in Biosensors: Should You Use Nanotubes or Graphene? [J].

Yang, Wenrong ;

Ratinac, Kyle R. ;

Ringer, Simon P. ;

Thordarson, Pall ;

Gooding, J. Justin ;

Braet, Filip .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (12) :2114-2138

[49] Real-time DNA detection using Pt nanoparticle-decorated reduced graphene oxide field-effect transistors [J].

Yin, Zongyou ;

He, Qiyuan ;

Huang, Xiao ;

Zhang, Juan ;

Wu, Shixin ;

Chen, Peng ;

Lu, Gang ;

Chen, Peng ;

Zhang, Qichun ;

Yan, Qingyu ;

Zhang, Hua .

NANOSCALE, 2012, 4 (01) :293-297

[50] Tailoring Electronic Properties of Graphene by π-π Stacking with Aromatic Molecules [J].

Zhang, Zengxing ;

Huang, Helin ;

Yang, Xiaomei ;

Zang, Ling .

JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2011, 2 (22) :2897-2905

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