Attomolar Label-Free Detection of DNA Hybridization with Electrolyte-Gated Graphene Field-Effect Transistors

被引：164

作者：

Campos, Rui ^{[1
,4
]}

Borme, Jerome ^{[1
]}

Guerreiro, Joana Rafaela ^{[2
]}

Machado, George, Jr. ^{[1
,3
]}

Cerqueira, Maria Fatima ^{[1
,3
]}

Petrovykh, Dmitri Y. ^{[2
]}

Alpuim, Pedro ^{[1
,3
]}

机构：

[1] Int Iberian Nanotechnol Lab, Dept Quantum & Energy Mat, P-4715330 Braga, Portugal

[2] Int Iberian Nanotechnol Lab, Dept Life Sci, P-4715330 Braga, Portugal

[3] Univ Minho, Ctr Phys, P-4710057 Braga, Portugal

[4] AXES Res Grp, Chem Dept, Groenenborgerlaan 171, B-2020 Antwerp, Belgium

来源：

ACS SENSORS | 2019年 / 4卷 / 02期

关键词：

biosensor; graphene; DNA; EGFET (electrolyte-gated field-effect transistor); planar technology; receded gate transistor; surface functionalization; ELECTRICAL DETECTION; GOLD; ALKANETHIOLS; BINDING; AMPLIFICATION; FABRICATION; MONOLAYERS; BIOSENSORS; NANOTUBES; SENSORS;

D O I：

10.1021/acssensors.8b00344

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this work, we develop a field-effect transistor with a two-dimensional channel made of a single graphene layer to achieve label-free detection of DNA hybridization down to attomolar concentration, while being able to discriminate a single nucleotide polymorphism (SNP). The SNP-level target specificity is achieved by immobilization of probe DNA on the graphene surface through a pyrene-derivative heterobifunctional linker. Biorecognition events result in a positive gate voltage shift of the graphene charge neutrality point. The graphene transistor biosensor displays a sensitivity of 24 mV/dec with a detection limit of 25 aM: the lowest target DNA concentration for which the sensor can discriminate between a perfect-match-target sequence and SNP-containing one.

引用

页码：286 / 293

页数：15

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