An enzymatic method for assaying tazobactam in vitro. Comparison with high performance liquid chromatography.

被引：0

作者：

Sotto, A ^{[1
]}

Brunschwig, C ^{[1
]}

Ramuz, M ^{[1
]}

Jourdan, J ^{[1
]}

机构：

[1] FAC MED,INSERM U431,F-30900 NIMES,FRANCE

来源：

PATHOLOGIE BIOLOGIE | 1997年 / 45卷 / 01期

关键词：

beta-lactamases; tazobactam; beta-lactamase inhibitors; nitrocefin;

D O I：

暂无

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

SUMM. We developed an enzymatic method using nitrocefin to assay tazobactam in vitro. Tazobactam was incubated with TEM-1 beta-lactamase. Then, residual beta-lactamase activity was assayed by adding nitrocefin. This activity corresponded indirectly to the initial concentration of tazobactam. Within-assay, between-assay and accuracy coefficients of variation were below 15%. The correlation coefficient between enzymatic method and high performance liquid chromatography (the reference method) was 0.98. The enzymatic method is rapid, easy to perform and should be applied to daily clinical practice.

引用

页码：77 / 81

页数：5

共 16 条

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AUGEY V, IN PRESS J CHROMATOG

[2] RAPID METHOD FOR DETECTING BETA-LACTAMASE PRODUCING BACTERIA IN CLINICAL SPECIMENS [J].