Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human γδ T cells in Escherichia coli

被引：305

作者：

Hintz, M

Reichenberg, A

Altincicek, B

Bahr, U

Gschwind, RM

Kollas, AK

Beck, E

Wiesner, J

Eberl, M

Jomaa, H

机构：

[1] Jomaa Pharmaka GMBH, D-35392 Giessen, Germany

[2] Univ Frankfurt, D-60590 Frankfurt, Germany

[3] Univ Marburg, Fachbereich Chem, NMR Abt, D-35043 Marburg, Germany

[4] Univ Giessen, Fachbereich Humanmed, Biochem Inst, D-35392 Giessen, Germany

来源：

FEBS LETTERS | 2001年 / 509卷 / 02期

关键词：

gamma delta T cell; 2-C-methyl-D-erythritol 4-phosphate pathway; 4-hydroxy-3-methyl-but-2-enyl pyrophosphate; gcpE; lytB;

D O I：

10.1016/S0014-5793(01)03191-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gcpE and lytB gene products control the terminal steps of isoprenoid biosynthesis via the 2-C-methyl-D-erythritol 4-phosphate pathway in Escherichia coli. In lytB-deficient mutants, a highly immunogenic compound accumulates significantly, compared to wild-type E. coli, but is apparently absent in gcpE-deficient mutants. Here, this compound was purified from E. coli Delta lytB mutants by preparative anion exchange chromatography, and identified by mass spectrometry, H-1, C-13 and P-31 NMR spectroscopy, and NOESY analysis as (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP). HMB-PP is 10(4) times more potent in activating human V gamma9/V delta2 T cells than isopentenyl pyrophosphate. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：317 / 322

页数：6

相关论文

共 35 条

[1] Structure of a human γδ T-cell antigen receptor [J].

Allison, TJ ;

Winter, CC ;

Fournié, JJ ;

Bonneville, M ;

Garboczi, DN .

NATURE, 2001, 411 (6839) :820-824

[2] Cutting edge:: Human γδ T cells are activated by intermediates of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis [J].

Altincicek, B ;

Moll, J ;

Campos, N ;

Foerster, G ;

Beck, E ;

Hoeffler, JF ;

Grosdemange-Billiard, C ;

Rodríguez-Concepción, M ;

Rohmer, M ;

Boronat, A ;

Eberl, M ;

Jomaa, H .

JOURNAL OF IMMUNOLOGY, 2001, 166 (06) :3655-3658

[3] LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli [J].

Altincicek, B ;

Kollas, AK ;

Eberl, M ;

Wiesner, J ;

Sanderbrand, S ;

Hintz, M ;

Beck, E ;

Jomma, H .

FEBS LETTERS, 2001, 499 (1-2) :37-40

[4] GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli [J].

Altincicek, B ;

Kollas, AK ;

Sanderbrand, S ;

Wiesner, J ;

Hintz, M ;

Beck, E ;

Jomaa, H .

JOURNAL OF BACTERIOLOGY, 2001, 183 (08) :2411-2416

[5] 3-formyl-1-butyl pyrophosphate A novel mycobacterial metabolite-activating human γδ T cells [J].

Belman, C ;

Espinosa, E ;

Poupot, R ;

Peyrat, MA ;

Guiraud, M ;

Poquet, Y ;

Bonneville, M ;

Fournié, JJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (45) :32079-32084

[6] The role of lateral gene transfer in the evolution of isoprenoid biosynthesis pathways [J].

Boucher, Y ;

Doolittle, WF .

MOLECULAR MICROBIOLOGY, 2000, 37 (04) :703-716

[7] Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli [J].

Campos, N ;

Rodríguez-Concepción, M ;

Seemann, M ;

Rohmer, M ;

Boronat, A .

FEBS LETTERS, 2001, 488 (03) :170-173

[8] Deuterium-labelled isotopomers of 2-C-methyl-D-erythritol as tools for the elucidation of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis [J].

Charon, L ;

Hoeffler, JF ;

Pale-Grosdemange, C ;

Lois, LM ;

Campos, N ;

Boronat, A ;

Rohmer, M .

BIOCHEMICAL JOURNAL, 2000, 346 :737-742

[9] A total synthesis of the unique tris-oxazole macrolide ulapualide A produced by the marine nudibranch Hexabranchus sanguineus [J].

Chattopadhyay, SK ;

Pattenden, G .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 2000, (15) :2429-2454

[10] STIMULATION OF HUMAN GAMMA-DELTA T-CELLS BY NONPEPTIDIC MYCOBACTERIAL LIGANDS [J].

CONSTANT, P ;

DAVODEAU, F ;

PEYRAT, MA ;

POQUET, Y ;

PUZO, G ;

BONNEVILLE, M ;

FOURNIE, JJ .

SCIENCE, 1994, 264 (5156) :267-270

← 1 2 3 4 →