Structural characterization and antioxidant activities of polysaccharides extracted from Epimedium acuminatum

被引:171
作者
Cheng, Haoran [1 ]
Feng, Shiling [1 ]
Jia, Xuejing [1 ]
Li, Qianqian [1 ]
Zhou, Yonghong [2 ]
Ding, Chunbang [1 ]
机构
[1] Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Sichuan, Peoples R China
[2] Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Sichuan, Peoples R China
关键词
Epimedium acuminatum Franch; Polysaccharide; Extraction; Structure; Antioxidant activities; HERBAL MEDICINAL INGREDIENTS; LYMPHOCYTE-PROLIFERATION; ASSISTED EXTRACTION; INHIBITION;
D O I
10.1016/j.carbpol.2012.09.051
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
assisted extraction, enzyme extraction, and microwave-assisted extraction. The physicochemical properties of Epimedium polysaccharides were then determined by chemical composition analysis, Fourier transform infrared (FT-IR) spectroscopy, and scanning electron microscopy (SEM) analysis. Further, the antioxidant activities were studied via different methods, including DPPH assay, ABTS assay, FRAP assay and AAPH-induced erythrocyte hemolysis assay. Results showed that the physicochemical properties of different polysaccharides were similar. Antioxidant assay indicated that four polysaccharides exhibited significant antioxidant activities in a dose-dependent manner. The antioxidant activities of the polysaccharides which obtained by hot water extraction were higher than those of other polysaccharides. Overall. E. acuminatum polysaccharides might be used as potential natural antioxidant. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:63 / 68
页数:6
相关论文
共 32 条
[11]   FREE RADICAL-DERIVED QUINONE METHIDE MEDIATES SKIN TUMOR PROMOTION BY BUTYLATED HYDROXYTOLUENE HYDROPEROXIDE - EXPANDED ROLE FOR ELECTROPHILES IN MULTISTAGE CARCINOGENESIS [J].
GUYTON, KZ ;
BHAN, P ;
KUPPUSAMY, P ;
ZWEIER, JL ;
TRUSH, MA ;
KENSLER, TW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (03) :946-950
[12]   OXYGEN-TOXICITY, OXYGEN RADICALS, TRANSITION-METALS AND DISEASE [J].
HALLIWELL, B ;
GUTTERIDGE, JMC .
BIOCHEMICAL JOURNAL, 1984, 219 (01) :1-14
[13]   Antioxidant activities of different fractions of polysaccharide purified from pulp tissue of litchi (Litchi chinensis Sonn.) [J].
Kong, Fan-Li ;
Zhang, Ming-Wei ;
Kuang, Rui-Bin ;
Yu, Shu-Juan ;
Chi, Jian-Wei ;
Wei, Zhen-Cheng .
CARBOHYDRATE POLYMERS, 2010, 81 (03) :612-616
[14]   Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken [J].
Kong, XF ;
Hu, YL ;
Rui, R ;
Wang, DY ;
Li, XG .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2004, 4 (07) :975-982
[15]   Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro [J].
Kovacevic, Nada ;
Colic, Miodrag ;
Backovic, Aleksandar ;
Doslov-Kokorus, Zvezdana .
FITOTERAPIA, 2006, 77 (7-8) :561-567
[16]  
Kumar C. G., 2004, ENZYM MICROB TECHNOL, V34, P673, DOI DOI 10.1016/J.ENZMICTEC.2004.03.001
[17]   Antioxidant flavonoids from Epimedium wushanense [J].
Li, Hui-fang ;
Guan, Xiang-yu ;
Yang, Wen-zhi ;
Liu, Ke-di ;
Ye, Min ;
Sun, Chao ;
Lu, Shuang ;
Guo, De-an .
FITOTERAPIA, 2012, 83 (01) :44-48
[18]   Evaluation of the antioxidant effects of polysaccharides extracted from Lycium barbarum [J].
Li, X. L. ;
Zhou, A. G. .
MEDICINAL CHEMISTRY RESEARCH, 2007, 15 (09) :471-482
[19]   Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation [J].
Lin, Jin-Yuarn ;
Tang, Ching-Yin .
FOOD CHEMISTRY, 2007, 101 (01) :140-147
[20]   Sulfated modification of epimedium polysaccharide and effects of the modifiers on cellular infectivity of IBDV [J].
Lu, Yu ;
Wang, Deyun ;
Hu, Yuanliang ;
Huang, Xiaoyan ;
Wang, Junmin .
CARBOHYDRATE POLYMERS, 2008, 71 (02) :180-186