Structural characterization and antioxidant activities of polysaccharides extracted from Epimedium acuminatum

被引：171

作者：

Cheng, Haoran ^{[1
]}

Feng, Shiling ^{[1
]}

Jia, Xuejing ^{[1
]}

Li, Qianqian ^{[1
]}

Zhou, Yonghong ^{[2
]}

Ding, Chunbang ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Sichuan, Peoples R China

[2] Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Sichuan, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2013年 / 92卷 / 01期

关键词：

Epimedium acuminatum Franch; Polysaccharide; Extraction; Structure; Antioxidant activities; HERBAL MEDICINAL INGREDIENTS; LYMPHOCYTE-PROLIFERATION; ASSISTED EXTRACTION; INHIBITION;

D O I：

10.1016/j.carbpol.2012.09.051

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

assisted extraction, enzyme extraction, and microwave-assisted extraction. The physicochemical properties of Epimedium polysaccharides were then determined by chemical composition analysis, Fourier transform infrared (FT-IR) spectroscopy, and scanning electron microscopy (SEM) analysis. Further, the antioxidant activities were studied via different methods, including DPPH assay, ABTS assay, FRAP assay and AAPH-induced erythrocyte hemolysis assay. Results showed that the physicochemical properties of different polysaccharides were similar. Antioxidant assay indicated that four polysaccharides exhibited significant antioxidant activities in a dose-dependent manner. The antioxidant activities of the polysaccharides which obtained by hot water extraction were higher than those of other polysaccharides. Overall. E. acuminatum polysaccharides might be used as potential natural antioxidant. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：63 / 68

页数：6

共 32 条

[1] Immuno stimulating activity of the hot water-soluble polysaccharide extracts of Anacyclus pyrethrum, Alpinia galanga and Citrullus colocynthis [J].

Bendjeddou, D ;

Lalaoui, K ;

Satta, D .

JOURNAL OF ETHNOPHARMACOLOGY, 2003, 88 (2-3) :155-160

[2] The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].

Benzie, IFF ;

Strain, JJ .

ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76

[3] A MODIFIED URONIC ACID CARBAZOLE REACTION [J].

BITTER, T ;

MUIR, HM .

ANALYTICAL BIOCHEMISTRY, 1962, 4 (04) :330-&

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu [J].

Chen, Ruizhan ;

Liu, Zhiqiang ;

Zhao, Jimin ;

Chen, Ruiping ;

Meng, Fanlei ;

Zhang, Min ;

Ge, Wencheng .

FOOD CHEMISTRY, 2011, 127 (02) :434-440

[6]

Chu YueFeng Chu YueFeng, 2006, Agricultural Sciences in China, V5, P873, DOI 10.1016/S1671-2927(06)60138-7

[7] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8] Effects of drying methods on the antioxidant activities of polysaccharides extracted from Ganoderma lucidum [J].

Fan, Liuping ;

Li, Jinwei ;

Deng, Kequan ;

Ai, Lianzhong .

CARBOHYDRATE POLYMERS, 2012, 87 (02) :1849-1854

[9] Epimedium polysaccharide and propolis flavone can synergistically stimulate lymphocyte proliferation in vitro and enhance the immune responses to ND vaccine in chickens [J].

Fan, Yunpeng ;

Hu, Yuanliang ;

Wang, Deyun ;

Guo, Zhenhuan ;

Zhao, Xiaona ;

Guo, Liwei ;

Zhao, Biao ;

Zhang, Jing ;

Wang, Yuanlei ;

Nguyen, The Luong .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2010, 47 (02) :87-92

[10] Polysaccharides from fruit calyx of Physalis alkekengi var. francheti: Isolation, purification, structural features and antioxidant activities [J].

Ge, Yu ;

Duan, Yufeng ;

Fang, Guozhen ;

Zhang, Yan ;

Wang, Shuo .

CARBOHYDRATE POLYMERS, 2009, 77 (02) :188-193

← 1 2 3 4 →