THE REGULATION OF ANGIOGENESIS BY MICRORNA-210-MEDIATED HIF-lα/VEGF SIGNALING PATHWAYS AFTER RENAL ISCHEMIA/REPERFUSION INJURY

Neovascularization is a major physiological response to ischemia, but the regulatory mechanism of renal angiogenesis after ischemic injury remains to be undetermined. MicroRNAs are a group of small non-coding RNAs with modulator activity of gene expression. This study aimed to observe the expression of microRNA-210-mediated HIF-l alpha/VEGF signaling pathways and explore the regulatory mechanism of the angiogenesis after ischemia-reperfusion (I/R) injury. Quantitative Real-Time RT-PCR analysis showed that miR-210, HIF-l alpha mRNA and VEGFmRNA in IR 4h and ld group were increased significantly compared to sham-operated control group. Western blot analysis also showed that HIF-l alpha protein in IR 4h and ld group were increased apparently compared to sham-operated control group. (n=5 each,* P<0. 05 vs. control) Thus, we conclude that The renal ischemia/reperfusion injury might put an influence on the expression of miR-210, HIF-la, VEGF. The regulation of angiogenesis after renal ischemia/reperfusion injury might be associated with the microRNA-210-mediated HIF-la/VEGF signaling pathways.