Site-Directed Mutagenesis

被引：46

作者：

Bachman, Julia ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

来源：

LABORATORY METHODS IN ENZYMOLOGY: DNA | 2013年 / 529卷

关键词：

D O I：

10.1016/B978-0-12-418687-3.00019-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational modifications, or to silence enzymatic activity. Alternatively, noncoding changes are often used to generate rescue constructs that are resistant to knockdown via RNAi.

引用

页码：241 / 248

页数：8