Ginkgetin induces apoptosis via activation of caspase and inhibition of survival genes in PC-3 prostate cancer cells

被引：45

作者：

You, Ok Heui ^{[1
]}

Kim, Sun-Hee ^{[1
]}

Kim, Bonglee ^{[1
]}

Sohn, Eun Jung ^{[1
]}

Lee, Hyo-Jeong ^{[1
]}

Shim, Bum-Sang ^{[1
]}

Yun, Miyong ^{[1
]}

Kwon, Byung-Mog ^{[2
]}

Kim, Sung-Hoon ^{[1
]}

机构：

[1] Kyung Hee Univ, Coll Korean Med, Seoul 130701, South Korea

[2] Univ Sci & Technol, Lab Chem Biol & Genom, Korea Res Inst Biosci & Biotechnol, Taejon 305806, South Korea

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2013年 / 23卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

Ginkgetin; PC-3 prostate cancer; Caspase-3; PARP; Sub-G1; Apoptosis; BILOBA LEAVES; BCL-2; BIFLAVONE; MITOCHONDRIA; KAEMPFEROL; RESISTANCE; MECHANISMS; GROWTH; DEATH;

D O I：

10.1016/j.bmcl.2013.02.080

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Ginkgetin is a natural biflavonoid isolated from leaves of Ginkgo biloba L. Though it was known to have anti-inflammatory, anti-influenza virus, anti-fungal activity, osteoblast differentiation stimulating activity and neuro-protective effects, the underlying antitumor mechanism of ginkgetin still remains unclear. Thus, in the present study, anti-cancer mechanism of ginkgetin was elucidated in human prostate cancer PC-3 cells. Ginkgetin suppressed the viability of PC-3 cells in a concentration-dependent manner and also significantly increased the sub-G1 DNA contents of cell cycle in PC-3 cells. Ginkgetin activated caspase-3 and attenuated the expression of survival genes such as Bcl-2, Bcl-x(L), survivin and Cyclin D1 at protein and mRNA levels. Consistently, pan-caspase inhibitor Z-DEVD-fmk blocked sub G1 accumulation and cleavages of PRAP and caspase 3 induced by ginkgetin in PC-3 cells. Overall, these findings suggest that ginkgetin induces apoptosis in PC-3 cells via activation of caspase 3 and inhibition of survival genes as a potent chemotherapeutic agent for prostate cancer treatment. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：2692 / 2695

页数：4

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