Increased apoptotic activity on inflammatory human placentas in spontaneous abortions during the first and second trimester of gestation: a histochemical and immunohistochemical study

被引：0

作者：

Deftereou, Theodora-Eleftheria ^{[1
]}

Lambropoulou, Maria ^{[1
]}

Chatzaki, Ekaterini ^{[2
]}

Koffa, Maria ^{[3
]}

Ypsilantis, Petros ^{[4
]}

Pagonopoulou, Olga ^{[5
]}

Grammatikopoulou, Iordana ^{[1
]}

Papadopoulos, Elias ^{[1
]}

Papalambros, Alexandros ^{[1
]}

Kontomanolis, Emmanouil ^{[6
]}

Papadopoulos, Nikolas ^{[1
]}

机构：

[1] Democritus Univ Thrace, Lab Histol Embryol, Dragana 68100, Alexandroupolis, Greece

[2] Democritus Univ Thrace, Pharmacol Lab, Dragana 68100, Alexandroupolis, Greece

[3] Democritus Univ Thrace, Dept Mol Biol, Dragana 68100, Alexandroupolis, Greece

[4] Democritus Univ Thrace, Lab Expt Surg & Surg Res, Dragana 68100, Alexandroupolis, Greece

[5] Democritus Univ Thrace, Physiol Lab, Dragana 68100, Alexandroupolis, Greece

[6] Democritus Univ Thrace, Dept Gynecol & Obstet, Dragana 68100, Alexandroupolis, Greece

来源：

FOLIA HISTOCHEMICA ET CYTOBIOLOGICA | 2012年 / 50卷 / 01期

关键词：

placenta; apoptosis; immunohistochemistry; congenital disorders; chorioamnionitis; DNA FRAGMENTATION; FETAL MEMBRANES; CELL-DEATH; CHORIOAMNIONITIS; ACTIVATION; CASPASE; RUPTURE; TROPHOBLASTS; MECHANISMS; PATHWAYS;

D O I：

10.5603/FHC.2012.0017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to investigate the role of apoptotic markers on inflammatory human placentas from spontaneous abortions during the first and second trimester of gestation and compare them to those without inflammation. Paraffin-embedded specimens from 76 placentas were investigated by conventional histology and immunohistochemistry using monoclonal antibodies against M30, Caspase 3, Caspase 8 and Caspase 9, as well as the terminal deoxynucleotidyl tranferase-mediated deoxyuridine triphosphate nick end labeling method. A higher prevalence of expression of apoptotic markers (94.4%) was observed in placentas associated with chorioamnionitis in comparison with those without inflammation. Our observations confirm that apoptosis is strikingly prevalent in placentas diagnosed with histologic chorioamnionitis, while the inflammation induces cell death. (Folia Histochemica et Cytobiologica 2012, Vol. 50, No. 1, 118-124)

引用

页码：118 / 124

页数：7

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