Purification, characterization and immunostimulatory activity of polysaccharide from Cipangopaludina chinensis

被引：31
作者：
Xiong, Qingping [1 ]
Jiao, Yunpeng [2 ]
Zhao, Xirong [1 ]
Chen, Xiaoming [1 ]
Zhang, Qianghua [1 ]
Jiang, Changxing [1 ]
机构：
[1] Huaiyin Inst Technol, Coll Life Sci & Chem Engn, Huaian 223003, Jiangsu, Peoples R China
[2] Jiangsu Food Sci Coll, Dept Food Engn, Huaian 223003, Jiangsu, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2013年 / 98卷 / 01期
关键词：
Purification; Preliminary characterization; Immunostimulatory activity; Cipangopaludina chinensis; IN-VIVO; STRUCTURAL-CHARACTERIZATION; IMMUNOMODULATORY ACTIVITY; IMMUNOSUPPRESSION; COMPONENTS; PROTECTS;
D O I：
10.1016/j.carbpol.2013.05.075
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In this study, we investigated the purification, preliminary characterization and immunostimulatory activity in vivo of polysaccharide from Cipangopaludina chinensis (CCPS). Firstly, crude CCPS was prepared by hot water extraction. And the crude CCPS was sequentially purified by chromatography of DEAE-52 and Sephadex G-100, resulting in two purified fractions of CCPS-1 and CCPS-2. We found the two fractions were homogeneous heteropolysaccharides mainly composed of rhamnose and glucose with the average molecular weight of 226 and 235 kDa, respectively. CCPS-2 was quite different from CCPS-1. It had much higher content of uronic acid and sulfuric radical. For immunostimulatory activity in vivo, crude CCPS could significantly increase the thymus and spleen indices, enhance the macrophage function, and increase the level of serum hemolysin in cyclophosphamide-treated mice, suggesting CCPS had a potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：217 / 223
页数：7
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