Improvement of Bacillus thuringiensis δ-endotoxins synthesis yields through acquisition of erythromycin resistance

被引：3

作者：

Tounsi, S ^{[1
]}

Zouari, N ^{[1
]}

Ghribi, D ^{[1
]}

J'mal, A ^{[1
]}

Jaoua, S ^{[1
]}

机构：

[1] Ctr Biotechnol Sfax, Lab Biopesticides, Sfax 3038, Tunisia

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 05期

关键词：

delta-endotoxins; Bacillus thuringiensis; cry gene; erythromycin resistance; sporulation;

D O I：

10.1007/s10529-005-5717-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The acquisition of the erythromycin resistance by Bacillus thuringiensis kurstaki improved yields of delta-endotoxins in sporulating cells ranging from 134 to 215%. Resistance to erythromycin decreased the final spore count by at least 50%. Consequently, erythromycin resistance is an efficient tool for the improvement of bioinsecticides yields with a high ratio of delta-endotoxins to spores.

引用

页码：315 / 319

页数：5

共 15 条

[11] Sambrook J, 1989, MOL CLONING LAB MANU
[12] Bacillus thuringiensis and its pesticidal crystal proteins
Schnepf, E
Crickmore, N
Van Rie, J
Lereclus, D
Baum, J
Feitelson, J
Zeigler, DR
Dean, DH
[J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) : 775 - +
[13] ERYTHROMYCIN RESISTANT MUTATIONS IN BACILLUS-SUBTILIS CAUSE TEMPERATURE SENSITIVE SPORULATION
TIPPER, DJ
JOHNSON, CW
GINTHER, CL
LEIGHTON, T
WITTMANN, HG
[J]. MOLECULAR AND GENERAL GENETICS, 1977, 150 (02): : 147 - 159
[14] Response of larval Ephesita kuehniella (Lepidoptera: Pyralidae) to individual Bacillus thuringiensis kurstaki toxins and toxin mixtures
Tounsi, S
Dammak, M
Rebaî, A
Jaoua, S
[J]. BIOLOGICAL CONTROL, 2005, 35 (01) : 27 - 31
[15] Characterization of a novel cry2Aa-type gene from Bacillus thuringiensis subsp kurstaki
Tounsi, S
Jaoua, S
[J]. BIOTECHNOLOGY LETTERS, 2003, 25 (15) : 1219 - 1223

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