A simple separation method for (S)-hydroxynitrile lyase from cassava and its application in asymmetric cyanohydrination

被引：9

作者：

Zheng, Zubiao ^{[1
]}

Zi, Yan ^{[1
]}

Li, Zhongzhou ^{[1
]}

Zou, Xinzhuo ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

TETRAHEDRON-ASYMMETRY | 2013年 / 24卷 / 08期

关键词：

OPTICALLY-ACTIVE CYANOHYDRINS; HYDROXYNITRILE LYASE; CONVENIENT SYNTHESIS; (R)-OXYNITRILASE; (R)-CYANOHYDRINS; (S)-CYANOHYDRINS; OXYNITRILASES; CATALYSIS; ALDEHYDES; ALMOND;

D O I：

10.1016/j.tetasy.2013.03.015

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Using an acetone precipitation method, crude (S)-hydroxynitrile lyase [(S)-MeHNL] was separated from Munihot esculenta (cassava) leaves, and used directly as biocatalyst to catalyze asymmetric cyanohydrination and produce cyanohydrins with enantiomeric purities (>= 90% ee) significantly greater than those previously reported. The use of a water/i-Pr2O system with an enzyme, NaCN, and appropriate amounts of acetic acid is crucial in improving the stereoselectivity of cyanohydrin formation by minimizing the non-enzymatic reaction and the racemization of the chiral products. The proposed isolation method for crude (S)-MeHNL has a high value because of its simplicity, and low cost as well as the high activity of the crude (S)-MeHNL. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：434 / 439

页数：6

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