共 25 条
Differential CD 146 expression on circulating versus tissue endothelial cells in rectal cancer patients: Implications for circulating endothelial and progenitor cells as biomarkers for antiangiogenic therapy
被引:103
作者:

Duda, DG
论文数: 0 引用数: 0
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机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA

Cohen, KS
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h-index: 0
机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA

di Tomaso, E
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h-index: 0
机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA

Au, AP
论文数: 0 引用数: 0
h-index: 0
机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA

Klein, RJ
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h-index: 0
机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA

Scadden, DT
论文数: 0 引用数: 0
h-index: 0
机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA

Willett, CG
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h-index: 0
机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA

Jain, RK
论文数: 0 引用数: 0
h-index: 0
机构: Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA
机构:
[1] Massachusetts Gen Hosp, Dept Radiat Oncol, Steele Lab Tumor Biol, Boston, MA 02114 USA
[2] Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02114 USA
[3] Harvard Univ, Sch Med, Boston, MA USA
[4] Duke Univ, Med Ctr, Dept Radiat Oncol, Durham, NC USA
关键词:
D O I:
10.1200/JCO.2005.04.2861
中图分类号:
R73 [肿瘤学];
学科分类号:
100214 ;
摘要:
Purpose Circulating endothelial cells (CECs) and progenitor cells are currently evaluated as potential biomarkers of antiangiogenic therapy. CD146 is considered a panendothelial-specific marker, but its utility as a CEC marker in cancer patients remains unclear. Patients and Methods We analyzed the expression of CD146 on mononuclear blood cells, primary tissue endothelial cells, and malignant and normal tissues by flow cytometric and immunohistochemical analyses. Furthermore, we measured the circulation kinetics of CD146(+) cells before, and then 3 and 12 days after vascular endothelial growth factor (VEGF) antibody blockade by bevacizumab infusion in rectal cancer patients enrolled in a phase I trial. Results In the peripheral blood of these cancer patients, over 90% of the CD146(+) cells were CD45(+) hematopoietic cells. CD146 expression was primarily detected on a subset of CD3(+)CD4(+) lymphocytes, and was undetectable on CD34(+)CD133(+)CD45(dim) progenitor cells or CD31(bright)CD45(-) viable CECs. In contradistinction, CD146 was detectable in tissues on both cellular components of tumor vessel wall: CD31(bright)CD45(-) endothelial cells and alpha-SMA(+) pericytes. Unlike viable CECs and progenitor cells, CD146(+) cell concentration in the peripheral blood of cancer patients did not decrease during VEGF blockade. Conclusion CD146 is fairly homogeneously expressed on vascular endothelium but not on viable CECs or progenitor cells. The vast majority of CD146(+) blood cells are lymphocytes, and VEGF blockade by bevacizumab did not significantly change their number in rectal cancer patients. These results underscore the need for further characterization and identification of new markers for CEC subpopulations for their development as biomarkers of antiangiogenic therapy.
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页码:1449 / 1453
页数:5
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