Effects of cadmium on antioxidant enzyme activities in sugar cane

被引：128
作者：
Fornazier, RF
Ferreira, RR
Vitória, AP
Molina, SMG
Lea, PJ
Azevedo, RA [1 ]
机构：
[1] Univ Sao Paulo, Escola Superior Agr Luiz Queiroz, Dept Genet, BR-13400970 Piracicaba, SP, Brazil
[2] Univ Lancaster, Dept Sci Biol, Lancaster LA1 4YQ, England
来源：
BIOLOGIA PLANTARUM | 2002年 / 45卷 / 01期
关键词：
catalase; glutathione reductase; heavy metals; phytochelatins; reactive oxygen species; Saccharum officinarum; superoxide dismutase;
D O I：
10.1023/A:1015100624229
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Sugar cane (Saccharum officinarum L, cv. Copersucar SP80-3280) seedlings were grown in nutrient solution with varying concentrations (0, 2 and 5 mM) of cadmium chloride for 96 h. Leaves were analysed for catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) activities. Although a clear effect of CdCl2 on plant growth was observed, the activity of SOD was riot altered significantly. However, the CAT activity decreased as the concentration of CdCl2 increased. GR exhibits a significant increase in activity at 2 and 5 mM CdCl2. CAT and SOD isoenzymes were further characterised by analysis in non-denaturing PAGE. Activity staining for SOD revealed up to seven isoenzymes in untreated control and 2 mM CdCl2 treated plants, corresponding to Cu/Zn-SOD isoenzymes. At 5 mM CdCl2, only six Cu/Zn-SOD isoenzymes were observed. No Fe-SOD and Mn-SOD isoenzymes were detected. For CAT, one band of activity was observed.
引用
收藏
页码：91 / 97
页数：7
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