Sequence specificity of the 8-hydroxyguanine repair activity in rat organs

被引：7
作者：
Hirano, T
Hirano, H
Yamaguchi, R
Asami, S
Tsurudome, Y
Kasai, H [1 ]
机构：
[1] Univ Occupat & Environm Hlth, Dept Environm Oncol, Kitakyushu, Fukuoka 8078555, Japan
[2] Univ Occupat & Environm Hlth, Dept Biochem, Kitakyushu, Fukuoka 8078555, Japan
来源：
JOURNAL OF RADIATION RESEARCH | 2001年 / 42卷 / 03期
关键词：
8-hydroxyguanine; DNA repair; oxygen radicals; endonuclease nicking assay; 8-oxoguanine DNA glycosylase 1;
D O I：
10.1269/jrr.42.247
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The base excision repair system for 8-hydroxyguanine (8-OH-Gua) is believed to play a role in the prevention of mutations, such as GC-to-TA transversion, which leads to cancer development. However, the exact repair mechanism is still unclear. In this study, we examine whether the repair activity level for 8-hydroxyguanine, one of the major forms of oxidative DNA damage, depends on the sequence of the substrate DNA. We prepared six different oligonucleotides containing 8-bydroxyguanine as substrates and reacted them with crude extracts from the livers and kidneys of 8-week-old Sprague-Dawley rats. As a result, up to a 10-fold difference in the repair activity levels was observed, depending on the substrates used. Based on this observation, we suggest that the repair systems may act with sequence specificity on the damaged DNA.
引用
收藏
页码：247 / 254
页数：8
相关论文
共 23 条
[1]  
Asami S, 1996, CANCER RES, V56, P2546
[2]   Cigarette smoking induces an increase in oxidative DNA damage, 8-hydroxydeoxyguanosine, in a central site of the human lung [J].
Asami, S ;
Manabe, H ;
Miyake, J ;
Tsurudome, Y ;
Hirano, T ;
Yamaguchi, R ;
Itoh, H ;
Kasai, H .
CARCINOGENESIS, 1997, 18 (09) :1763-1766
[3]   Purification and characterization of a novel DNA repair enzyme from the extremely radioresistant bacterium Rubrobacter radiotolerans [J].
Asgarani, E ;
Terato, H ;
Asagoshi, K ;
Shahmohammadi, HR ;
Ohyama, Y ;
Saito, T ;
Yamamoto, O ;
Ide, H .
JOURNAL OF RADIATION RESEARCH, 2000, 41 (01) :19-34
[4]   SYNTHESIS OF 2'-DEOXY-7,8-DIHYDRO-8-OXOGUANOSINE AND 2'-DEOXY-7,8-DIHYDRO-8-OXOADENOSINE AND THEIR INCORPORATION INTO OLIGOMERIC DNA [J].
BODEPUDI, V ;
SHIBUTANI, S ;
JOHNSON, F .
CHEMICAL RESEARCH IN TOXICOLOGY, 1992, 5 (05) :608-617
[5]  
CHENG KC, 1992, J BIOL CHEM, V267, P166
[6]  
CHUNG MH, 1991, BIOCHEM BIOPH RES CO, V188, P1
[7]   Repair of oxidative DNA damage in Drosophila melanogaster:: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines [J].
Dherin, C ;
Dizdaroglu, M ;
Doerflinger, H ;
Boiteux, S ;
Radicella, JP .
NUCLEIC ACIDS RESEARCH, 2000, 28 (23) :4583-4592
[8]   Stimulation of human 8-oxoguanine-DNA glycosylase by AP-endonuclease: potential coordination of the initial steps in base excision repair [J].
Hill, JW ;
Hazra, TK ;
Izumi, T ;
Mitra, S .
NUCLEIC ACIDS RESEARCH, 2001, 29 (02) :430-438
[9]   AGE-ASSOCIATED CHANGE OF 8-HYDROXYGUANINE REPAIR ACTIVITY IN CULTURED HUMAN FIBROBLASTS [J].
HIRANO, T ;
YAMAGUCHI, Y ;
HIRANO, H ;
KASAI, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 214 (03) :1157-1162
[10]   8-Hydroxyguanine levels in nuclear DNA and its repair activity in rat organs associated with age [J].
Hirano, T ;
Yamaguchi, R ;
Asami, S ;
Iwamoto, N ;
Kasai, H .
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 1996, 51 (05) :B303-B307
123